Purification, structure characterization of bioactive substances from marine organisms and application them to novel anti-oral cancer drugs with iPS cell research technique

• Project/Area Number: 26463006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Yukio YOSHIOKA 広島大学, 医歯薬保健学研究院(歯), 助教 (20335665)
• Co-Investigator(Kenkyū-buntansha): 岡本 哲治 広島大学, 医歯薬保健学研究院(歯), 教授 (00169153) ; 山崎 佐知子 広島大学, 病院(歯), 病院助教 (00632001) ; 小鹿 一 名古屋大学, 生命農学研究科, 教授 (50152492)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 海洋生物 / 細胞毒性 / 抗腫瘍効果 / 扁平上皮癌細胞 / 無血清培養 / ヒトiPS細胞 / 創薬 / crambescidin 657 / Laurencia intricata / 細胞毒性試験 / Aplystatin / PalisadinA / Palisol / 生理活性物質 / 扁平上皮癌 / iPS細胞

Outline of Final Research Achievements

Marine organisms have some different systems of metabolism and physiological functions from the living thing on the ground. We extract the bioactive substances from them and isolate the substances with the strong antitumor activity for the index of the cytotoxicity against cancer cells. We aim not only to analyze the antitumor effect for oral squamous cell carcinoma cells but also apply them to the novel antitumor agents against oral cancer. Marine organisms from both adjacent Japanese sea and adjacent Indonesian sea are collected and freeze-dried. The extracted compounds are investigated their activity by cytotoxic assay for cancer cells and are purified.The novel bioactive substances are investigated with the toxicity for the human iPS cells which are differentiated into some important organic cells under the serum free culture.

Research Products

• [Journal Article] Weekly paclitaxel plus cetuximab reduces the lung metastasis of adenoid cystic carcinoma arising from the salivary gland (2015)
  • Author(s): Yukio YOSHIOKA, Shigeaki TORATANI, Hirotaka NAKATAO; Kouichi KOIZUMI, Yasutaka HAYASHIDO, Tetsuji OKAMOTO
  • Journal Title: Oral Science International Volume: 12 Issue: 2 Pages: 67-71
  • DOI: 10.1016/s1348-8643(15)00019-1
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 海洋生物由来生理活性物質の探索研究 (2016)
  • Author(s): 吉岡幸男，小鹿一，岡本哲治
  • Organizer: 第70回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 福岡市
  • Year and Date: 2016-04-15
• [Presentation] PaclitaxelとCetuximab併用療法により抗腫瘍効果を認めた口蓋腺様嚢胞癌肺転移の一例 (2014)
  • Author(s): 吉岡幸男，鷹津冬良，福井康人，小泉浩一，谷亮治，林堂安貴，虎谷茂昭，藤高一慶，岡本哲治ら
  • Organizer: 第４３回（公社）日本口腔外科学会 中四国支部学術集会
  • Place of Presentation: 徳島大学
  • Year and Date: 2014-04-25