The analysis of the role of CCN3 in the formation of vascularization at the tissue regeneration and the constraction of the drug discovery

• Project/Area Number: 26463017
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Nagasaki University
• Principal Investigator: MINAMIZATO Tokutaro 長崎大学, 医歯薬学総合研究科(歯学系), 客員研究員 (50529807)
• Co-Investigator(Kenkyū-buntansha): 山口 朗 東京歯科大学, 歯学部, 客員教授 (00142430) ; 朝比奈 泉 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30221039)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 再生医療

Outline of Final Research Achievements

Several lines of evidence have demonstrated that the CCN family of proteins regulates differentiation of the skeletalmesenchymal cells such as muscle cells, chondrocytes and osteoblasts. In this research, for the purpose of the anlysis of function of CCN3 in the formation of vascularization in tissue regeneration, we focus on the interation between CCN3 and VEGF, CD34 cells, especially. As the results, we verified that CCN3 controled the formation of new vascularization by binding to the VEGF which is secreted protein, experimentally. In addition, we demonstratd that the CCN3-Notch pathway also regulate the new vascularization, indirectly.

Research Products

• [Journal Article] Bone regeneration using Dentin Matrix depends on the degree of demineralization and particle size (2016)
  • Author(s): Takamitsu Koga, Tokutaro Minamizato, Yosuke Kawai, Kei-ichiro Miura, Takashi I, Yuya Nakatani, Yoshinori Sumita, Izumi Asahina:
  • Journal Title: PLOS ONE Volume: 11(1）
  • Peer Reviewed / Open Access
• [Journal Article] The skeletal and dentoalveolar stability of the maxilla after LeFort I osteotomy for cant correction. (2016)
  • Author(s): Ohba S, Nakao N, Nakatani Y, Minamizato T, Kawasaki T, Koga T, Matsuura T, Yoshimura H, Yoshida N, Sano K, Asahina I
  • Journal Title: Cranio Volume: 34 Pages: 38-42
  • Peer Reviewed / Open Access
• [Journal Article] Skeletal stability after sagittal splitting ramus osteotomy with physiological positioning strategy in skeletal mandibular prognathism with facial asymmetry. (2016)
  • Author(s): Seigo Ohba, Noriko Nakao, Takako Kawasaki, Kei-ichiro Miura, Tokutaro Minamizato, Yoshiyuki Koga, Noriaki Yoshida, Izumi Asahina
  • Journal Title: British Journal of Oral and Maxillofacial Surgery Volume: 54(8) Pages: 920-926
  • Peer Reviewed / Open Access
• [Journal Article] Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size (2016)
  • Author(s): Koga T, Minamizato T, Kawai Y, Miura K, I T, Nakatani Y, Sumita Y, Asahina I
  • Journal Title: Plos One Volume: 11
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Bone Marrow-derived Cell Therapy for Oral Mucosal Repair after Irradiation (2014)
  • Author(s): 14.I T, Sumita Y, Minamizato T, Umebayashi M, Liu Y, Tran SD, Asahina I
  • Journal Title: Journal of Dental Research Volume: 30;93(8) Pages: 813-820
  • Peer Reviewed / Open Access