| Project/Area Number |
26463019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岸田 昭世 鹿児島大学, 医歯学域医学系, 教授 (50274064)
岐部 俊郎 鹿児島大学, 医歯学域附属病院, 助教 (50635480)
岸田 想子 鹿児島大学, 医歯学域医学系, 助教 (40274089)
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| Co-Investigator(Renkei-kenkyūsha) |
KIBE Toshiro 鹿児島大学, 医歯学域歯学系, 助教 (50635480)
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| Research Collaborator |
KISHIDA Michiko 鹿児島大学, 医歯学域医学系, 助教 (40274089)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 歯原性腫瘍 / 上皮-間葉相互作用 / エナメル上皮腫 / 骨浸潤 / エナメル上皮腫上皮腫 / インターロイキン / 腫瘍間質 / 線維芽細胞 / 相互作用 |
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| Outline of Final Research Achievements |
Analyses of the interaction, cross-talk, between ameloblstoma cell (AM-3) and fibroblast cell (HFF-2) revealed that AM-3 secreted IL-α and facilitated of IL-6 and IL-8 secretion of HFF-2 fibroblasts, and stimulation by the cocultured supernatant of both cells accelerated the migration activity of AM-3. These findings suggest that the ameloblastoma cells and stromal fibroblasts behave interactively via cytokines to create a microenvironment that leads to the extension of ameloblastomas.
Three dimensional observation using double layered collagen gel hemisphere culture revealed that AM-3 cells formed tuft-like invasive processes and collectively invaded into outer layer. These findings were more apparent under the presence of HEF-2 fibroblasts when compared to the single culture of AM-3. HFF-2 fibroblasts localized to the tips of the invasive tumor processes, suggesting that tumor-associated cells assist tumor cell invasion.
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