| Project/Area Number |
26463021
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Yamamoto Toshiro 京都府立医科大学, 医学(系)研究科(研究院), 講師 (40347472)
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| Co-Investigator(Kenkyū-buntansha) |
雨宮 傑 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90398389)
喜多 正和 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (60153087)
金村 成智 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (70204542)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 培養シート / 歯髄由来細胞 / 羊膜 / 骨分化誘導 / 培養細胞シート |
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| Outline of Final Research Achievements |
In the present study, we developed an osteoblast differentiation-inducing dental pulp-derived cell sheet on a human amniotic membrane by culturing the cell-membrane complex in a medium supplemented with osteoblast-inducer reagent. Implantation of the osteoblast differentiation-inducing dental pulp-derived cell sheet on the dorsal skin of nude mice led to the formation of calcified tissue. Similarly, implantation of the osteoblast differentiation-inducing sheet on periodontal bone defects in rats led to a significant increase in bone formation compared with the control sheet of cells cultured in a control medium.
These findings suggest that the osteoblast differentiation-inducing dental pulp-derived cell sheet on a human amniotic membrane may be useful for the regeneration of periodontal tissue.
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