| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
Human dedifferentiated fat (hDFAT) cells are thought to be a promising cell source for cartilage regeneration therapy. Nevertheless, the responses of hDFAT cells to bone morphogenetic proteins (BMPs) are still unclear. Recombinant BMP-4 (100 ng/mL) increased the expression of SOX9, SOX6, and aggrecan mRNAs in monolayer cells compared with that in cells treated with BMP-2 or BMP-7 on day 3. Chondrogenically differentiated hDFAT cells induced by CM containing BMP-4 showed higher expression of eight genes in monolayer cultures and nine genes in pellet cultures compared with those in control medium on day 14. Dorsomorphin attenuated the effects of BMP-4. These results showed that BMP-4 had the potential to modulate the early chondrogenesis of hDFAT cells under both monolayer and pellet cell culture conditions.
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