Pharmaceutical therapeutic assesment for temporomanidibular disorders by using sinovitis model of human TMJ in vitro

• Project/Area Number: 26463034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: KAKUDO Kenji 大阪歯科大学, 歯学部, 名誉教授 (30131379)
• Co-Investigator(Renkei-kenkyūsha): NAKATA Ken 大阪大学, 医学研究科(研究院), 教授 (00283747)
• Research Collaborator: KONDO Mayo ; YATANI Shinya ; OKAMOTO Tomoko
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 顎関節症 / 薬物療法 / 滑膜炎 / PRP / 顎関節 / 滑膜細胞 / 多血小板血漿 / ヒト / 滑膜炎モデル / 抗炎症性鎮痛薬 / 三次元培養 / 圧縮負荷 / 金属プロテアーゼ

Outline of Final Research Achievements

When clenching the teeth, compressive force is applied directly to the synovium in the disc, causing synovitis of temporomandibular disorders (TMJ disc derangement). We have developed a novel synovitis model using a cyclic loading bioreactor, and cultured human synovial cells in a 3D cell-scaffold construct. We studied pharmaceutical agents for temporomandibular disorders by using this model. This system can asses the effects of pharmaceutical agents for TMD in vitro.

Research Products

• [Journal Article] Effect of platelet-rich plasma on proliferation of human synovial cells (2016)
  • Author(s): Shinya Yatani, Ken Nakata,Kenji Kakudo
  • Journal Title: Journal of Osaka Dental University Volume: 50 Issue: 1 Pages: 31-35
  • DOI: 10.18905/jodu.50.1_31
  • NAID: 130005166042
  • ISSN: 0475-2058, 2189-6488
  • Peer Reviewed / Open Access
• [Presentation] The effect of platelet rich plasma(PRP) on the human synovial cells in vitro (2016)
  • Author(s): Kenji Kakudo, Shinya Yatani, Ken Nakata
  • Organizer: 23rd Congress of the European Association for Cranio Maxillo-Facial Surgery
  • Place of Presentation: London (UK)
  • Year and Date: 2016-09-13
  • Int'l Joint Research
• [Presentation] ヒト滑膜三次元培養組織へのメカニカルストレスとIL1β，IL6刺激によるPGE2発現上昇 (2015)
  • Author(s): 岡本知子，矢谷真也，金銅真世，室井悠里，覚道健治，中田 研，赤峯勇哲
  • Organizer: 第60回日本口腔外科学会総会・学術大会
  • Place of Presentation: 名古屋国際会議場（愛知県，名古屋市）
  • Year and Date: 2015-10-16
• [Presentation] 多血小板血漿はヒト滑膜細胞の増殖を促進する (2015)
  • Author(s): 矢谷真也，中田 研，覚道健治
  • Organizer: 第28回日本顎関節学会総会・学術大会
  • Place of Presentation: 名古屋国際会議場（愛知県，名古屋市）
  • Year and Date: 2015-07-04
• [Presentation] 平面培養における健常/OA由来軟骨細胞の遺伝子発現 (2015)
  • Author(s): 矢谷真也，武 靖浩，岡本知子，河野史倫，米谷泰一，中井直也，中田 研，覚道健治
  • Organizer: 第69回日本口腔科学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府，大阪市）
  • Year and Date: 2015-05-13
• [Presentation] 三次元培養ヒト滑膜炎モデルにおける MMPs遺伝子発現に及ぼす抗炎症薬の影響. (2014)
  • Author(s): 金銅真世, 岡本知子, 矢谷真也, 覚道健治.
  • Organizer: 第59回日本口腔外科学会総会・学術大会
  • Place of Presentation: 千葉市 幕張メッセ
  • Year and Date: 2014-10-17
• [Presentation] ヒト滑膜三次元培養組織への繰り返し力学負荷における発現遺伝子の時間的経過. (2014)
  • Author(s): 岡本知子, 矢谷真也, 金銅真世, 赤峯勇哲, 室井悠里, 覚道健治.
  • Organizer: 第59回日本口腔外科学会総会・学術大会
  • Place of Presentation: 千葉市 幕張メッセ
  • Year and Date: 2014-10-17