Development of gene therapy for oral cancer with direct injection of Naked DNA of alpha2-antiplasmin gene

• Project/Area Number: 26463044
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: HAMANA TOMOAKI 広島大学, 医歯薬保健学研究院(歯), 助教 (40397922)
• Co-Investigator(Kenkyū-buntansha): 岡本 哲治 広島大学, 医歯薬保健学研究院(歯), 教授 (00169153) ; 林堂 安貴 広島大学, 病院(歯), 講師 (70243251)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: α2-アンチプラスミン / プラスミン / E-カドヘリン / Naked DNA / 口腔癌遺伝子治療

Outline of Final Research Achievements

SCCKN and alpha2-AP transfectants were subcutaneously inoculated into the nude mice. E-cadherin on cell membrane of xenograft from alpha2-AP transfectants was increased compared to SCCKN. Moreover, beta-catenin increased on cell membrane of xenograft from alpha2-AP transfectants.
These findings suggest that the suppression of the plasminogen activator/plasmin system by alpha2-AP might reduce the dissemination of OSCC cells. Therefore, it could be expected the development of gene therapy for invasiveness and metastasis of OSCC cells with induction of alpha2-AP into OSCC tissue.

Research Products

• [Journal Article] Prostate Sphere-forming Stem Cells Are Derived from the P63-expressing Basal Compartment (2015)
  • Author(s): Yanqing Huang, Tomoaki Hamana, Junchen Liu, Cong Wang, Lei An, Pan You, Julia Y. F. Chang, Jianming Xu , Wallace L. McKeehan, and Fen Wang
  • Journal Title: J Biol Chem Volume: 290 Pages: 17745-52
  • Peer Reviewed
• [Journal Article] Type 2 fibroblast growth factor receptor signaling preserves stemness and prevents differentiation of prostate stem cells from the basal compartment (2015)
  • Author(s): Yanqing Huang, Tomoaki Hamana, Junchen Liu, Cong Wang, Lei An, Pan You, Julia Y. F. Chang, Jianming Xu , Chengliu Jin, Zhongying Zhang, Wallace L. McKeehan, and Fen Wang
  • Journal Title: J Biol Chem Volume: 290 Pages: 17753-61
  • Peer Reviewed
• [Journal Article] Overexpression of FGF9 in Prostate Epithelial Cells Augments Reactive Stroma Formation and Promotes Prostate Cancer Progression (2015)
  • Author(s): Yanqing Huang, Chengliu Jin, Tomoaki Hamana, Junchen Liu, Cong Wang, Lei An, Wallace L. McKeehan, and Fen Wang
  • Journal Title: Int. J. Biol. Sci. Volume: 11 Pages: 948-960
  • Peer Reviewed