| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Aiming at establishing a pair of autologous cancer cell line and its CTL for the identification of novel cancer antigen, Sapporo Medical University Hospital Dental Department of Oral Surgery than the biopsy tissue of cheek oral squamous cell carcinoma patient treated In the establishment of a cell line successfully, PBL or TIL of a patient with established cancer cell line was isolated, cultured in AIM-VRMedium supplemented with rI l -2 (50 IU / ml), and irradiated with X rays Mixed culture of autologous cancer cell line and PBL or TIL was repeated, induction of CTL succeeded in establishing pair of autologous cancer cell line and its CTL. Analysis of autologous cancer-specific cytotoxicity mechanism was carried out.
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