| Project/Area Number |
26463058
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Wakita Ryo 東京医科歯科大学, 大学院医歯学総合研究科, 准教授 (60376712)
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| Co-Investigator(Kenkyū-buntansha) |
船山 ひろみ 鶴見大学, 歯学部, 助教 (00359530)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 気管支平滑筋 / 漢方薬 / カルシウムイオン / trifolirhizin / ホスホキナーゼ / Caチャンネル / Trifolrhizin |
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| Outline of Final Research Achievements |
Pre-treatment of primary cultures of airway smooth muscle by trifolirhizin inhibited the increase of intracellular calcium ion concentration. However the activation of phosphokinase A was not observed in the airway smooth muscle cells pretreated with culture of airway epithelial cells which were exposed to herbal medicine, and continuing suppression of muscle contraction was not also observed. Therefore, the involvement of low calcium ion dependent pathway is suspected, and the use of phosphorylation of myosin light chain as an index of contractile is expected to further investigation.
Meanwhile we found the possibility that trifolirhizin can work by inhibiting the PKC pathway. In addition, the combination use of PC2-B and trifolirhizin obtained enhanced inhibition of intracellular calcium increase, so that alternative pathway like Rho is required to consider as a target.
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