Histological examination of bone regeneration and cell kinetics induced by mesenchymal stem cells: comparison of bone marrow cells with adipose tissue cells
| Project/Area Number |
26463060
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
Kojima Taku 新潟大学, 医歯学総合病院, 講師 (90515777)
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| Co-Investigator(Kenkyū-buntansha) |
芳澤 享子 松本歯科大学, 歯学部, 教授 (60303137)
小林 正治 新潟大学, 医歯学系, 教授 (80195792)
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| Co-Investigator(Renkei-kenkyūsha) |
AMIZUKA Norio 北海道大学, 大学院歯学総合研究科, 教授 (30242431)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 骨再生 / 間葉系幹細胞 / バイオマテリアル / 骨髄 / 脂肪 |
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| Outline of Final Research Achievements |
To aim for efficient bone regeneration, we employed tissue engineering which composed of mesenchymal stem cells and biomaterials. Therefore, we examined bone regeneration with bone marrow cells or adipose tissue cells/porous beta-TCP block composites by means of histological analysis. Bone marrow cells and adipose tissue cells obtained from 4-week-old GFP rat were induced into osteoblast-like cell, and then, seeded on a porous beta-TCP block. 10-week-old nude rats were implanted with the blocks in the calvariae, and then the blocks were extracted and examined histochemically. The differentiated bone marrow cells and adipose tissue cells have high osteogenic potential. Therefore, these results suggest that not only bone marrow cells but also adipose tissue cells have possibilities to be effective cell sources for bone regeneration.
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Report
(4 results)
Research Products
(3 results)
