Role of sirtuin in volatile anesthetic-induced cardiac protection.

• Project/Area Number: 26463071
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: KITAHATA Hiroshi 徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (60161486)
• Co-Investigator(Kenkyū-buntansha): 堤 保夫 徳島大学, 大学院医歯薬学研究部(医学系), 准教授 (90523499) ; 川人 伸次 徳島大学, 大学院医歯薬学研究部(医学系), 特任教授 (60284296) ; 高石 和美 徳島大学, 病院, 講師 (20325286)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 心筋保護作用 / Sirtuin / カベオリン / プレコンディショニング / イソフルラン / 吸入麻酔薬 / 虚血再灌流障害 / 細胞膜マイクロドメイン / 心筋保護 / 虚血再灌流 / サーチュイン / アポトーシス

Outline of Final Research Achievements

Using the mouse myocardial ischemia-reperfusion model, our results show that, volatile anesthetic-induced cardiac protection is dependent on an anti-aging gene, sirtuin, and a membrane microdomain, caveolin.
Additionally, our results suggest that caveolin within the heart are critical for sirtuin-induced cardiac protection via volatile anesthetics.

Research Products

• [Journal Article] Strict blood glucose control by an artificial endocrine pancreas during hepatectomy may prevent postoperative acute kidney injury (2017)
  • Author(s): Naoji Mita, Shinji Kawahito, Tomohiro Soga, Kazumi Takaishi, Hiroshi Kitahata, Munehide Matsuhisa, Mitsuo Shimada, Hiroyuki Kinoshita, Yasuo M Tsutsumi, Katsuya Tanaka
  • Journal Title: Journal of Artificial Organs Volume: 1 Issue: 1 Pages: 76-83
  • DOI: 10.1007/s10047-016-0925-6
  • Peer Reviewed
• [Journal Article] Isoflurane-induced postconditioning via mitochondrial calcium-activated potassium channels (2016)
  • Author(s): Michiko Kinoshita, Yasuo M. Tsutsumi, Kohei Fukuta, Asuka Kasai, and Katsuya Tanaka.
  • Journal Title: The Journal of Medical Investigation Volume: 63 Issue: 1.2 Pages: 80-84
  • DOI: 10.2152/jmi.63.80
  • NAID: 130005141048
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed / Open Access
• [Journal Article] Effects of oral carbohydrate with amino acid solution on the metabolic status of patients in the preoperative period: a randomized, prospective clinical trial. (2016)
  • Author(s): Tsutsumi R, Kakuta N, Kadota T, Oyama T, Kume K, Hamaguchi E, Niki N, Tanaka K, Tsutsumi YM
  • Journal Title: Journal of Anesthesia Volume: 30 Issue: 5 Pages: 842-849
  • DOI: 10.1007/s00540-016-2217-y
  • Peer Reviewed / Open Access
• [Journal Article] C-terminal region of GADD34 regulates eIF2α dephosphorylation and cell proliferation in CHO-K1 cells. (2016)
  • Author(s): Otsuka R, Harada N, Aoki S, Shirai K, Nishitsuji K, Nozaki A, Hatakeyama A, Shono M, Mizusawa N, Yoshimoto K, Nakaya Y, Kitahata H, Sakaue H
  • Journal Title: Cell stress and chaperones Volume: 21 Issue: 1 Pages: 2940-2940
  • DOI: 10.1007/s12192-015-0633-9
  • Peer Reviewed
• [Journal Article] Exendin-4, glucagon-like peptide-1 receptor agonist, enhances isoflurane-induced preconditioning against myocardial infarction via caveolin-3 expression. (2015)
  • Author(s): Hamaguchi E, Tanaka K, Tsutsumi R, Sakai Y, Fukuta K, Kasai A, Tsutsumi YM
  • Journal Title: European Review for Medical and Pharmacological Sciences Volume: 19 Pages: 12851290-12851290
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Impact of newly developed, next-generation artificial endocrine pancreas. (2015)
  • Author(s): Kambe N, Kawahito S, Mita N, Takaishi K, Katayama T, Sakai Y, Soga T, Kawano H, Matsushita M, Shimada M, Kitagawa T, Kitahata H
  • Journal Title: The Journal of Medical Investigation Volume: 62 Pages: 41-44
  • NAID: 130005129688
  • Peer Reviewed
• [Journal Article] Geranylgeranylacetone and volatile anesthetic-induced cardiac protection synergism is dependent on caveolae and caveolin-3. (2014)
  • Author(s): Tsutsumi YM, Tsutsumi R, Horikawa YT, Sakai Y, Hamaguchi E, Kitahata H, Kasai A, Kambe N, Tanaka K
  • Journal Title: Journal of Anesthesia Volume: 28 Pages: 733-739
  • Peer Reviewed
• [Presentation] シンポジウム「神経調節による循環制御」カリウムチャネルと循環 (2016)
  • Author(s): 川人伸次，曽我朋宏，箕田直治，堤 保夫，田中克哉，高石和美，北畑 洋，木下浩之
  • Organizer: 第37回日本循環制御医学会総会
  • Place of Presentation: ステーションコンファレンス東京（東京都千代田区）
  • Year and Date: 2016-07-08
  • Invited
• [Presentation] イソフランによる心筋保護作用はSirtによるOpa-1制御を介する (2016)
  • Author(s): 堤 保夫，香留希実子，濵口英佑，酒井陽子，北畑 洋，田中克哉
  • Organizer: 日本麻酔科学会第63回学術集会
  • Place of Presentation: マリンメッセ福岡（福岡県福岡市）
  • Year and Date: 2016-05-26
• [Presentation] Leucine induces cardioprotection by promoting mitochondrial fusion via mTOR and Opa-1 signaling in virto and diet induced obese mice. (2015)
  • Author(s): Tsutsumi R, Tsutsumi Y, Roth D, Patel H, Tanaka K, Sakaue H
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Westin Miyako Kyoto (Kyoto・Kyoto)
  • Year and Date: 2015-10-28
  • Int'l Joint Research
• [Presentation] Blood glucose control by artificial endocrine pancreas during hepatectomy prevents postoperative acute kidney injury. (2015)
  • Author(s): Mita,N, Kawahito S, Soga T, Tanaka K, Takaishi, K, Kitahata H
  • Organizer: The Annual Meeting of the American Society of Anesthesiologists
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2015-10-27
  • Int'l Joint Research
• [Presentation] Role of sirtuins in cardioprotection by ischemic and anesthetic preconditioning. (2015)
  • Author(s): Kitahata H, Tsutsumi Y, Aoyama T, Takaishi K, Kawahito S, Tanaka K
  • Organizer: Euroanaesthesia 2015, The European Anaesthesiology Congress
  • Place of Presentation: Berlin (Germany)
  • Year and Date: 2015-06-01
  • Int'l Joint Research
• [Presentation] Role of sirtuins in cardioprotection by ischemic and anesthetic preconditioning. (2015)
  • Author(s): Kitahata H, Tsutsumi Y, Aoyama T, Takaishi K, Kawahito S, Tanaka K
  • Organizer: Euroanaesthesia 2015
  • Place of Presentation: Berlin (Germany)
  • Year and Date: 2015-06-01
• [Presentation] Effects of beta-3 adrenergic stimulation on nitric oxide production in myocardial cells of neonatal rats. (2015)
  • Author(s): Takaishi K, Kawahito S, Soga T, Otsuka R, Tsutsumi Y, Kitahata H
  • Organizer: Euroanaesthesia 2015, The European Anaesthesiology Congress
  • Place of Presentation: Berlin (Germany)
  • Year and Date: 2015-05-31
  • Int'l Joint Research
• [Presentation] The effects of intravenous anesthetics on cell migration using cultured human umbilical vein endothelial cells. (2015)
  • Author(s): Takaishi K, Kawahito S, Mita N, Aoyama T, Otsuka R, Kitahata H
  • Organizer: IARS 2015 Annual Meeting and International Science Symposium
  • Place of Presentation: Honolulu, Hawaii (USA)
  • Year and Date: 2015-03-22