The study of jaw bone regeneration therapy with the complex of PRF, SHAp, and ASCs

Research Project

Project/Area Number	26463077
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Surgical dentistry
Research Institution	Kochi University
Principal Investigator	YOSHIZAWA YASUMASA 高知大学, 教育研究部医療学系臨床医学部門, 助教 (90433812)
Co-Investigator(Kenkyū-buntansha)	近藤誠二昭和大学, 歯学部, 准教授 (10432634) 椋代義樹昭和大学, 歯学部, 助教 (50325099)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	ハイドロキシナノアパタイト / 脂肪幹細胞 / PRF / スキャフォールド / 再生医学 / ナノハイドロキシアパタイト / 骨再生
Outline of Final Research Achievements	①We have successfully purified ASCs from human fat tissue, and three-dimensionally cultured ASCs into mixture of SHAp and PRF. After that, we comprehensively investigated the expression of bone-related genes and proteins with molecular biological analysis. ② We have transplanted the complex of SHAp, PRF and ASCs. After that, we histologically investigated the bone regeneration in vivo.