The study of bone modeling signals mediated by periodontal stem cells under mechanical stress

• Project/Area Number: 26463089
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KANEKO Sawa 東京医科歯科大学, 歯学部, 非常勤講師 (80372449)
• Co-Investigator(Kenkyū-buntansha): 細道 純 東京医科歯科大学, 大学院医歯学総合研究科, 講師 (00420258) ; 石田 雄之 東京医科歯科大学, 学内共同利用施設等, 特任助教 (00516297) ; 清水 康広 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (60631968) ; 臼見 莉沙 東京医科歯科大学, 歯学部附属病院, 医員 (90706946)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯の矯正移動 / 動物実験 / 歯根膜幹細胞 / 矯正的歯の移動 / 動物モデル / Ki67 / LGR5 / 歯根膜細胞 / 歯根膜 / 矯正移動 / リモデリング / 咬合刺激 / 歯周組織 / メカニカルストレス / デコイ / 低酸素

Outline of Final Research Achievements

The response of stem cell-associated markers to orthodontic force in the periodontal ligament (PDL) in vivo is poorly understood. The aim of the study was to characterize the expression and localization of cell proliferation and osteogenic differentiation factors in the PDL during tooth movement, using Sprague-Dawley rats. Our findings suggested that orthodontic force induces Ki67 and LGR5-positive cells in the PDL in the early phase of orthodontic tooth movement.

Research Products

• [Presentation] Distribution of Ki67 and LGR5-positive Periodontal Cells During Tooth Movement (2017)
  • Author(s): Jun Hosomichi, Takumi Suzuki, Naoki Shibutani, Kasumi Hatano, Hiroyuki Yamaguchi, Yo-ichiro Kuma, Sawa Kaneko, Takashi Ono
  • Organizer: 2017 IADR/AADR/CADR General Session & Exhibition
  • Place of Presentation: サンフランシスコ（アメリカ合衆国）
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] Effect of Low-Intensity Pulsed Ultrasound on periodontal tissues in occlusal hypofunctional teeth (2016)
  • Author(s): Yuki Kasahara, Risa Usumi-Fujita, Jun Hosomichi, Sawa Kaneko, Yuji Ishida, Naoki Shibutani, Yasuhiro Shimizu, Asuka Okito, Shuji Oishi, Yoichiro Kuma, Takashi Ono
  • Organizer: 38th Annual Scientific Conference on Dental Research
  • Place of Presentation: ベトナム・ホーチミン市
  • Year and Date: 2016-04-04
  • Int'l Joint Research
• [Presentation] Introducing a decoy into the periodontium via an ultrasound microbubble. (2015)
  • Author(s): Yamaguchi H, Ishida Y, Suzuki J, Watanabe R, Hosomichi J, Shitano C, Usumi-Fujita R, Shimizu Y, Kaneko S, Isobe M, Ono T.
  • Organizer: 2015 IADR/AADR/CADR General Session & Exhibition
  • Place of Presentation: Boston (USA)
  • Year and Date: 2015-03-11 – 2015-03-14