A study of osteoblast differentiation and osteogenesis in transcription factor Msx2-transfer human iPS cells
| Project/Area Number |
26463098
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
白方 良典 鹿児島大学, 医歯学域歯学系, 准教授 (60359982)
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| Research Collaborator |
MITSUI Kaoru
KOSAI Kenichiro
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 骨芽細胞 / 細胞分化 |
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| Outline of Final Research Achievements |
There have been no clear reports so far that transcription factor Msx2 gene take part in the mechanism of osteoblast differentiation, which is responsible for the bone remodeling and regeneration in orthodontic treatments. The purpose of this study, therefore, was to investigate the roles of transcription factor Msx2 in osteoblast differentiation and osteogenesis with Induced Pluripotent Stem (iPS) cells. iPS cells which transfected with Msx2 gene were cocultured with feeder cells, and mRNA levels of osteoblastic phenotype and alkaline phosphatase activity were determined for osteoblast differentiation and osteogenesis. The value of bone nodules was also determined for mineralization. As a result, it was suggested that transcription factor Msx2 play a key role in controlling osteoblast differentiation from iPS cells.
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Report
(5 results)
Research Products
(2 results)
