The effect of low-power laser irradiation on DFAT differentiation

• Project/Area Number: 26463101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Nihon University
• Principal Investigator: SHIMIZU Noriyoshi 日本大学, 歯学部, 教授 (40154299)
• Co-Investigator(Kenkyū-buntansha): 馬谷原 琴枝 日本大学, 歯学部, 講師 (60440046)
• Research Collaborator: TSURUMACHI Niina ; SAKAGUCHI Masahito
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: DFAT / 骨形成促進作用 / 急速拡大 / 上顎正中口蓋縫合 / 脱分化脂肪細胞 / 正中口蓋縫合 / 骨形成促進 / マイクロＣＴ / ＬＬＬＴ / ＤＦＡＴ / 間葉系幹細胞

Outline of Final Research Achievements

As a result of setting conditions for isolating a large number of DFATs, it was found to be optimal to prepare DFAT from mature adipocyte fraction of less than 40 μm diameter, and to treat with 0.02% collagenase for adipose tissue separation.
In the transplantation experiment of DFAT injection into the rapid expansion site of rat maxillary mid-palatal suture using micro-CT images, there was a tendency to promote osteogenesis in the sutured area after DFAT injection compared with the control group, but there was no significant difference.

Research Products

• [Journal Article] Effect of collagenase concentration to isolate small adipocytes from human buccal fat pad (2017)
  • Author(s): Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T, Kazama T, Oki Y, Tamura Y, Tonogi N, Shimizu N, Honda M
  • Journal Title: Journal of Oral Science Volume: 印刷中
  • NAID: 130006539176
  • Peer Reviewed
• [Journal Article] Small buccal fat pad cells have high osteogenic differentiateon potential. (2016)
  • Author(s): Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T, Kazama T, Oki Y, Tamura Y,Tonogi M, Isokawa K, Shimizu N, Honda M
  • Journal Title: Tissue Engineering Part C: Methods Volume: 22 Issue: 3 Pages: 250-9
  • DOI: 10.1089/ten.tec.2015.0420
  • Peer Reviewed
• [Presentation] ヒト頬脂肪体から単離した各種成熟脂肪細胞から得られた脱分化脂肪細胞の特性の解析. (2015)
  • Author(s): 鶴町 仁奈，秋田 大輔，松本 太郎, 加野 浩一郎, 外木 守雄, 鳥海 拓, 磯川 桂太郎, 清水 典佳, 本田 雅規
  • Organizer: 第36回日本炎症再生医学会
  • Place of Presentation: 虎ノ門ヒルズフォーラム(東京港区）
  • Year and Date: 2015-07-21
• [Presentation] Differentiated fat cells isolated human buccal fat pad (2015)
  • Author(s): Niina Tsurumachi; Daisuke Akita ; Taro Matsumoto; Koichiro Kano; Morio Tonogi ; Eisuke Kawano ; Taku Toriumi; Keitaro Isokawa; Noriyoshi Shimizu; Masaki Honda
  • Organizer: International Association of Dental Reseach
  • Place of Presentation: Boston U.S.A
  • Year and Date: 2015-03-12