| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In present study, we attempted to identify growth plate chondrocyte-derived secretory factors using an efficient signal sequence trap (SST) method. An SST library from mouse rib cartilage growth plate cDNA was constructed and screened. We focused on secreted protein IGFBP5 and membrane protein FGFR5 among more than 70 of proteins identified by the screening. Then we found that these were expressed at specific stage during the chondrocyte differentiation stage. IGFBP5 was highly expressed on proliferating chondrocytes, and FGFR5 was on the middle of proliferating/hypertrophic stage.
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