Screening of secretion proteins in mouse growth plate chondrocytes
| Project/Area Number |
26463113
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
HIDAKA Kiyoshi 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10389421)
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| Co-Investigator(Kenkyū-buntansha) |
山本 雅哉 植草学園大学, 保健医療学部, 教授 (20446115)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 軟骨細胞分化 / 分泌性蛋白質 / IGFBP5 / FGFR5 |
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| Outline of Final Research Achievements |
In present study, we attempted to identify growth plate chondrocyte-derived secretory factors using an efficient signal sequence trap (SST) method. An SST library from mouse rib cartilage growth plate cDNA was constructed and screened. We focused on secreted protein IGFBP5 and membrane protein FGFR5 among more than 70 of proteins identified by the screening. Then we found that these were expressed at specific stage during the chondrocyte differentiation stage. IGFBP5 was highly expressed on proliferating chondrocytes, and FGFR5 was on the middle of proliferating/hypertrophic stage.
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Report
(5 results)
Research Products
(13 results)
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[Presentation] Construction of New Risk Predictive Model based on Machine Learning Method.2017
Author(s)
Kondo Y, Motomura Y, Murayama K, Nishimata H, Nishida K, Imamura K, Satoh K, Hidaka K, Kamasaki K, Nishiguchi M, Hoshino T, Fujiwara T
Organizer
10th Biennial Conference of the Pediatric Dentistry Association of Asia
Place of Presentation
東京ドームホテル（東京都・文京区）
Year and Date
2017-05-26
Related Report
Int'l Joint Research
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