Molecular differential inhibition mechanism of osteoclastic bone resorption by glucocorticoid-induced osteop orosis in childhood.

• Project/Area Number: 26463115
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: Maki Kenshi 九州歯科大学, 歯学部, 教授 (60209400)
• Co-Investigator(Kenkyū-buntansha): 自見 英治郎 九州大学, 歯学研究院, 教授 (40276598)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: osteoclast / p130cas / Bif-1 / PCR / Osteoporosis / 破骨細胞 / Rankl / p130Cas / SH3領域 / ステロイド / 骨粗鬆症 / NF-κB / RANKL / RelB / 骨粗しょう症 / 古典的経路 / ステロイド誘導性骨粗鬆症 / RelB欠損マウス

Outline of Final Research Achievements

Osteopetrosis due to dysfunction of osteoclasts in p130Cas-deficient mice suggest p130Cas play important roles for osteoclastic bone resorption. we focused Bif-1 which binding p130Cas because of the novelty, primary structure that can be involved in regulation of cytoskeleton. Western blotting and quantitative real-time PCR analysis showed the expression level of Bif-1 increased during osteoclastic differentiation. Bone mineral density of femurs isolated from Bif-1-deficient mice was higher than that of wild-type femurs using μCT analysis. These results suggest that Bif-1 is one of the candidate proteins to regulate osteoclastic bone resorption as a downstream molecule of p130Cas.

Research Products

• [Journal Article] High-fat diet-induced obesity triggers alveolar bone loss and spontaneous periodontal disease in growing mice (2016)
  • Author(s): Fujita Y, Maki K
  • Journal Title: BMC Obesity Volume: 3 Issue: 1 Pages: 1-1
  • DOI: 10.1186/s40608-016-0082-8
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effect of dietary calcium deficiency and altered diet hardness on the jawbone growth: a micro-CT and bone histomorphometric study in rats. (2016)
  • Author(s): Fujita Y, Goto S, Ichikawa M, Hamaguchi A, Maki K.
  • Journal Title: Archives of Oral Biology Volume: 72 Pages: 200-210
  • DOI: 10.1016/j.archoralbio.2016.08.036
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 7.Goto S, Fujita Y, Hotta M, Sugiyama A, Maki K. Influence of differences in the hardness and calcium content of diets on the growth of craniofacial bone in rats. (2015)
  • Author(s): Goto S, Fujita Y, Hotta M, Sugiyama A, Maki K
  • Journal Title: Angle Orhod Volume: 85 Pages: 969-79
  • Peer Reviewed / Int'l Joint Research
• [Presentation] A p130Cas binding protein Bif-1 is involved in osteoclastic bone resorption (2017)
  • Author(s): Kenya Touyama, MatsubaraTakuma, Soichiro Kokabu, Kenshii Maki, Eijiro Jimi
  • Organizer: Asia Pacific Conference
  • Int'l Joint Research
• [Presentation] BiologicalEffects of Ameloblastin and Enamelin on Bone Metabolism.1 (2016)
  • Author(s): Wichida Chaweewannakorn,Kenshi Maki,Wataru Ariyoshi, Toshinori Okinaga,Tatsuji Nishihara.
  • Organizer: 10th Binnial Confernce of the Pediatric Dentsitry Association of Asia
  • Place of Presentation: 東京
  • Year and Date: 2016-05-26
  • Int'l Joint Research
• [Presentation] 食餌のカルシウム含有量の違いおよび硬度の変化が顎骨の成長・発育に及ぼす影響 (2015)
  • Author(s): 2.後藤翔太, 藤田優子, 堀田舞佳, 杉山絢子, 高山幸美, 佐伯 桂, 牧 憲司
  • Organizer: 第53回日本小児歯科学会大会,
  • Place of Presentation: 広島
  • Year and Date: 2015-05-21
• [Presentation] 食物の硬軟およびカルシウム含有量の違いが顎骨の成長•発育に及ぼす影響 (2014)
  • Author(s): 後藤翔太、藤田優子、堀田舞佳、杉山絢子、牧憲司
  • Organizer: 日本小児歯科学会
  • Place of Presentation: 北九州市
  • Year and Date: 2014-11-23