| Project/Area Number |
26463120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
浅野 正岳 日本大学, 歯学部, 教授 (10231896)
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| Research Collaborator |
TAKEI Hiroki
ISHIYAMA Misa
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | Mecp2 / Gad1 / 延髄呼吸中枢 / ヒストンアセチル化 / バルプロ酸 / エピジェネティクス / 呼吸中枢 / プロモーター / CpGメチル化 / レット症候群 / バルプロ酸ナトリウム / MBD1 / MBD2 / McCP2 / 呼吸ニューロン |
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| Outline of Final Research Achievements |
Epigenetic effects of valproate (VPA) on Gad1 expression in the medullary ventral respiratory group (VRG) of neonatal Mecp2-deficient mice were examined. We found that the Gad1 mRNA level in the VRG of Mecp2-deficient mice was lower than that of wild-type mice while VPA treatment for 7 days increased Gad1 mRNA expression in the VRG of Mecp2-deficient mice on postnatal day 15. Acetylation levels of H3K9 and H4K5, the lysine residues of core histones, were increased with the VPA treatment in the Mecp2-deficient mice. These results suggest that the effects of VPA on Gad1 mRNA expression in the VRG of Mecp2-deficient mice are caused by the increase in the acetylation levels of core histones which may enhance Gad1 promoter activity.
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