Functional role of basement membrane organization using EL mice with tooth agenesis in the third molar

• Project/Area Number: 26463126
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tsurumi University
• Principal Investigator: Asada Yoshinobu 鶴見大学, 歯学部, 教授 (20184145)
• Co-Investigator(Kenkyū-buntansha): 伊平 弥生 鶴見大学, 歯学部, 学内講師 (40200018) ; 成山 明具美 鶴見大学, 歯学部, 助教 (90440304)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 基底膜分子 / ラミニン / インテグリン / ELマウス / 歯胚消失 / 歯胚 / LAMA5 / Integrinβ1 / SHH / 基底膜 / マウス / 歯胚の消失

Outline of Final Research Achievements

A detailed study using in situ hybridization and immunohistological analysis revealed that the absence of third molar (M3) in EL mice is strongly controlled by the interaction between integrin-α6β1 and laminin-5. Based on the results, it was indicated that abnormality of interaction between integrin-α6β1 and laminin-5 might induce abnormal expression of Sonic hedgehog (Shh) gene followed by aberration of Lymphoid enhancer-binding factor 1 (Lef1) and Paired box gene 9 (Pax9). These results suggest that integrin-α6β1 and laminin-5 constituting the basement membrane molecules play an important role in the development of the tooth germs of M3 in EL mice. We concluded that the tooth agenesis of M3 in EL mice is regulated by the basement membrane proteins such as integrin-α6β1 and laminin-5.

Research Products

• [Journal Article] Functions of miR-1 and miR-133a during the postnatal development of masseter and gastrocnemius muscles. (2015)
  • Author(s): 8.Nariyama M, Mori M, Shimazaki E, Ando H, Ohnuki Y, Abo T, Yamane A, Asada Y.
  • Journal Title: Mol Cell Biochem Volume: 407 Pages: 17-27
  • Peer Reviewed
• [Presentation] 歯胚の消失モデルマウスを用いた基底膜分子の機能的役割について (2017)
  • Author(s): 田島 格、伊平弥生、成山明具美、朝田芳信
  • Organizer: 第５５回日本小児歯科学会大会
  • Place of Presentation: 西日本総合展示場AIM３階(福岡県北九州市)
  • Year and Date: 2017-05-25
• [Presentation] 骨形成不全症Ⅱ型の乳歯象牙質の形態学的観察 (2014)
  • Author(s): 伊平弥生
  • Organizer: 日本小児歯科学会
  • Place of Presentation: 品川区立総合区民会館
  • Year and Date: 2014-05-17
• [Presentation] DLX3遺伝子多型とう蝕感受性との関連性 (2014)
  • Author(s): 太田増美
  • Organizer: 日本小児歯科学会
  • Place of Presentation: 品川区立総合区民会館
  • Year and Date: 2014-05-16