Prevention of preterm birth -effect of perodontal bacteria on placenta and umbilical cord-

• Project/Area Number: 26463128
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Katagiri Sayaka 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60510352)
• Co-Investigator(Kenkyū-buntansha): 小林 宏明 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (50396967) ; 宮坂 尚幸 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (70313252)
• Research Collaborator: MAEKAWA Shogo ; KOMAZAKI Rina ; UDAGAWA Sayuri ; SASAKI Naoki ; WATANABE Kazuki ; TAKEUCHI Yasuo ; SHIBA Takahiko ; OHTSH Anri
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 歯周病 / 低体重出産 / 早産 / 低体重児出産

Outline of Final Research Achievements

We examined periodontal bacteria in saliva, plaque and placenta among 47 threaten preterm labor (TPL) and healthy pregnant subjects. Almost all periodontopathic bacteria were found in saliva, plaque, and placenta samples. P. gingivalis in saliva and F. nucleatum in placenta tissue were significantly higher in the TPL group than in the healthy pregnant group.
Bacteremia was induced in pregnant Jcl:ICR mice (8-weeks-old) by intravenous injection of Pg. ED or control (CO) mice were injected Pg or saline Every-Day during pregnancy.Birth weight was lower for pups in the ED groups compared to those in the CO group.

Research Products

• [Journal Article] Overexpressing IRS1 in Endothelial Cells Enhances Angioblast Differentiation and Wound Healing in Diabetes and Insulin Resistance (2016)
  • Author(s): Katagiri S, Park K, Maeda Y, Rao TN, Khamaisi M, Li Q, Yokomizo H, Mima A, Lancerotto L, Wagers A, Orgill DP, King GL
  • Journal Title: Diabetes Volume: 65 Issue: 9 Pages: 2760-2771
  • DOI: 10.2337/db15-1721
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] PKCdelta inhibition normalizes the wound-healing capacity of diabetic human fibroblasts. (2016)
  • Author(s): Mogher Khamaishi, Sayaka Katagiri, Hillary Keenan, Kyoungmin Park, Yasutaka Maeda, Qian Li, Weier Qi, Thomas Thomou, Danielle Eschuk, Ana Tellechea, Aris Veves, Chenyu Huang, Dennis Pul Orgill, Amy Wagers, George L. King.
  • Journal Title: The Journal of Clinical Investigation Volume: 126(3) Issue: 3 Pages: 887-53
  • DOI: 10.1172/jci82788
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Bone Metabolic Microarray Analysis of Ligature-Induced Periodontitis in Streptozotocin-Induced Diabetic Mice (2016)
  • Author(s): Shogo Maekawa, Sayaka Katagiri, Yasuo Takeuchi, Rina Komazaki, Anri Ohtsu, Sayuri Udagawa, Yuichi Izumi.
  • Journal Title: Journal of Periodontal Research Volume: in press Issue: 2 Pages: 233-245
  • DOI: 10.1111/jre.12387
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Blue-violet light emitting diode (LED) irradiation in combination with hemostatic gelatin sponge (Spongel®) application ameliorates immediate socket bleeding in patients taking warfarin (2014)
  • Author(s): Okamoto T, Ishikawa I, Kumasaka A, Morita S, Katagiri S, Okano T, Ando T.
  • Journal Title: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology Volume: 117(2): Issue: 2 Pages: 170-177
  • DOI: 10.1016/j.oooo.2013.09.009
  • Peer Reviewed
• [Journal Article] Highprevalence of general and abdominal obesity and relationship of obesity with diabetes mellitus in patients with psychiatric disorders (2014)
  • Author(s): Kumiko Asahi, Nobuo Imazeki, Tosei Takahashi, Mari Takeuchi, Noriyo Kimura, Eriko Sasaki, Hideyuki Uchino, Kenji Murasugi, Akiko Hayakawa, Sayaka Katagiri, Noriko Ishizuka, Yoko Suzuki, Karin Sekiya, Misako Takahashi, Eiko Sato, Kiyoshi Kawakubo, Yoshio Uehara, Shinji Ikemoto,Shuji Inoue.
  • Journal Title: Journal of Diabetes and Obesity Volume: 1(2) Issue: 2 Pages: 1-6
  • DOI: 10.15436/2376-0494.14.011
  • Peer Reviewed / Open Access
• [Journal Article] Serine phosphorylation sites on IRS2 activated by angiotensin II and protein kinase C to induce selective insulin resistance in endothelial cells (2013)
  • Author(s): Park K, Li Q, Rask-Madsen C, Mima A, Mizutani K, Winnay J, Maeda Y, D'Aquino K, White MF, Feener EP, King GL
  • Journal Title: Mol Cell Biol Volume: 33 Issue: 16 Pages: 418-427
  • DOI: 10.1128/mcb.00506-13
  • Peer Reviewed
• [Presentation] Bone metabolic microarray analysis of ligature-nduced periodontitis in streptozotocin-induced diabetic mice. (2016)
  • Author(s): Maekawa S, Katagiri S, Takeuchi Y, Komazaki R, Ohtsu A, Udagawa S, Izumi Y.
  • Organizer: The 102nd annual meeting of American Academy of Periodontology.
  • Place of Presentation: San Diego, USA
  • Year and Date: 2016-09-10
• [Presentation] 糖尿病モデルマウスにおける結紮誘導歯周炎の解析 (2015)
  • Author(s): 前川祥吾、 片桐さやか、 駒崎利奈、 宇田川小百合、 大津杏理、 竹内康雄、 和泉雄一
  • Organizer: 第58回秋季日本歯周病学会学術大会
  • Place of Presentation: アクトシティ浜松、静岡県、浜松市
  • Year and Date: 2015-09-11