Functional analysis of microbiota associated with peri-implantitis and design the new treatment strategy

• Project/Area Number: 26463129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Takeuchi Yasuo 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60396968)
• Co-Investigator(Kenkyū-buntansha): 丸山 史人 京都大学, 医学研究科, 准教授 (30423122) ; 片桐 さやか 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (60510352)
• Co-Investigator(Renkei-kenkyūsha): KOYANAGI Tatsuro 東京医科歯科大学, 歯学部附属病院, 医員 (70632173)
• Research Collaborator: MARUYAMA Noriko 東京医科歯科大学, 歯学部附属病院, 医員 ; SHIBA Takahiko 東京医科歯科大学, 大学院・医歯(薬)学総合研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: インプラント周囲炎 / 歯周炎 / 歯周病原細菌 / トランスクリプトーム

Outline of Final Research Achievements

Metatranscriptomic analyses were performed to compare microbial species and functional genes in situ between peri-implantitis and periodontitis. Although the two diseases differed in terms of 16S rRNA-based taxonomic profiles, they showed similarities with respect to functional genes and taxonomic and virulence factor mRNA profiles. The latter, that was defined as microbial virulence types, differed from those of healthy periodontal sites. Networks based on co-occurrence relationships of taxonomic mRNA abundance (co-occurrence networks) were dissimilar between the two diseases. Peri-implantitis and periodontitis have shared as well as distinct microbiological characteristics.

Research Products

• [Journal Article] Active microbiota show specific correlationships in peri-implantitis and periodontitis (2017)
  • Author(s): Kachi H, Maruyama N, Maruyama F, Shiba T, Watanabe T, Goda A, Murase K, Michi Y, Takeuchi Y, Izumi Y, Harada K, Nakagawa I
  • Journal Title: The Journal of the Stomatological Society, Japan Volume: 84 Pages: 25-36
  • NAID: 40021162420
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Distinct interacting core taxa in co-occurrence networks enable discrimination of polymicrobial oral diseases with similar symptoms (2016)
  • Author(s): Shiba T, Watanabe T, Kachi H, Koyanagi T, Maruyama N, Murase K, Takeuchi Y, Maruyama F, Izumi Y, Nakagawa I
  • Journal Title: Scientific reports Volume: 6 Issue: 1 Pages: 30997-30997
  • DOI: 10.1038/srep30997
  • NAID: 120005841407
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 細菌叢遺伝子発現解析によるインプラント周囲炎および歯周炎の疾患特異的な細菌ネットワーク (2017)
  • Author(s): 渡辺孝康, 芝 多佳彦, 加地博一, 村瀬一典, 竹内康雄, 丸山史人, 和泉雄一, 中川一路
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2017-03-19
• [Presentation] Functional keystone species enable discrimination of peri-implantitis and periodontitis (2016)
  • Author(s): Shiba T, Watanabe T, Koyanagi T, Maruyama N, Takeuchi Y, Maruyama F, Izumi Y, Nakagawa I
  • Organizer: The 102nd Annual Meeting of the American Academy of Periodontology
  • Place of Presentation: San Diego Convention Center (San Diego, USA)
  • Year and Date: 2016-09-10
  • Invited
• [Presentation] インプラント周囲炎と歯周炎における細菌叢メタトランスクリプトーム解析 (2016)
  • Author(s): 渡辺孝康, 芝 多佳彦, 加地博一, 竹内康雄, 丸山史人, 和泉雄一, 中川一路
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-08-25
• [Presentation] 類似した臨床症状を呈する複合感染症から紐解く恒常性維持機構 (2016)
  • Author(s): 芝 多佳彦，渡辺孝康，小柳達郎，丸山緑子，竹内康雄，丸山史人，中川一路，和泉雄一
  • Organizer: 第59回日本歯周病学会春季学術大会
  • Place of Presentation: 宝山ホール（鹿児島県鹿児島市）
  • Year and Date: 2016-05-21
• [Presentation] Pyrosequencing analysis of subgingival microbiota in chronic periodontitis (2016)
  • Author(s): Ikeda E, Takeuchi Y, Suda W, Ikeda Y, Nakasato A, Hattori M, Izumi Y
  • Organizer: 45th Annual Meeting and Exhibition of the American Association for Dental Research
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2016-03-16
  • Int'l Joint Research
• [Presentation] Bacterial activity in peri-implantitis and periodontitis. (2015)
  • Author(s): Maruyama N, Goda A, Takeuchi Y, Shiba T, Koyanagi T, Izumi Y, Maruyama F, Nakagawa I
  • Organizer: Europerio 8 (European federation of periodontology)
  • Place of Presentation: London, UK
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] Inconsistency between abundance of 16S rRNA and mRNA in bacterial community of periodontitis and peri-implantitis. (2015)
  • Author(s): Shiba T, Watanabe F, Maruyama F, Takeuchi Y, Koyanagi T, Maruyama N, Nakagawa I, Izumi Y
  • Organizer: Europerio 8 (European federation of periodontology)
  • Place of Presentation: London, UK
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Remarks] プレス通知資料 「インプラント周囲炎の原因となる細菌群集構造を解明」
  • URL: http://www.tmd.ac.jp/archive-tmdu/kouhou/20160817_1.pdf