Molecular basis of amelogenin-induced periodontal tissue regeneration
| Project/Area Number |
26463137
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontology
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| Research Institution | Kyushu University |
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Principal Investigator |
Fukuda Takao 九州大学, 歯学研究院, 助教 (80507781)
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| Co-Investigator(Kenkyū-buntansha) |
西村 英紀 九州大学, 歯学研究院, 教授 (80208222)
讃井 彰一 九州大学, 大学病院, 講師 (70507780)
武富 孝治 久留米大学, 医学部, 助教 (10553290)
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| Co-Investigator(Renkei-kenkyūsha) |
UCHIUMI Takeshi 九州大学, 医学研究院, 准教授 (80253798)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | アメロジェニン / 歯周組織 / 再生 / GRP78 / 歯根膜幹細胞 / マクロファージ / Grp78 / 歯根膜 / Rac1 |
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| Outline of Final Research Achievements |
Amelogenin, the major component of enamel matrix derivative (Straumann® Emdogain), is well recognized in periodontology. It is used in periodontal surgery to regenerate cementum, periodontal ligament, and alveolar bone. However, the precise molecular mechanisms underlying periodontal regeneration are still unclear.In order to gain further insight into how amelogenin induces periodontal tissue regeneration, we conducted a protein interaction screen using recombinant full-length amelogenin (rM180) as bait. Using this proteomic screen we identified glucose-regulated protein 78 (Grp78) as an amelogenin-binding protein.our study demonstrates that the biological interaction of Grp78 with amelogenin enhances amelogenin-induced cell migration in PDLSCs. Our findings provide a better understanding of the molecular basis of amelogenin-induced periodontal regeneration.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Grp78 is critical for amelogenin-induced cell migration in a multipotent clonal human periodontal ligament cell line.2016
Author(s)
Toyoda K, Fukuda T, Sanui T*, Tanaka U, Yamamichi K, Atomura R, Maeda H, Tomokiyo A, Taketomi T, Uchiumi T, Nishimura F
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Journal Title
Journal of Cellular Physiology
Volume: 231
Issue: 2
Pages: 414-427
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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