Explore the possibility of novel periodontal therapy by regulating mitochondrial function of oral polymorphonuclear leucocytes.
Project/Area Number |
26463143
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Periodontology
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Research Institution | Ohu University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 口腔内好中球 / 活性酸素 / ミトコンドリア / アポトーシス / オートファジー / 抗酸化作用 / 細胞死 / 酸化ストレス / 好中球 / Bad |
Outline of Final Research Achievements |
The expression of the apoptosis inhibitor gene, FLIP mRNA, was examined from periodontitis gingiva by RT-PCR method, and it was possible to detect from approximately 40% of the samples. This result was consistent with the result that chronic inflammation persisted as a result of inhibition of cell death by apoptosis of lymphocytes infiltrating periodontitis gingiva. On the other hand, FLIP mRNA was not detected from the oral neutrophils, supporting the result that the expression level of pro-apoptotic Bad was high and the expression level of inhibitory phosphorylation was significantly low. The increased levels of IL-1 beta, IL-6 and IL-17 in GCF were interpreted as a result of inflammation and immune response in periodontal tissues. Both necrosis and apoptosis may be involved in the mechanism because neutrophils in oxidative stress have decreased intracellular glutathione concentration and caspase 8 activity.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Salivary pathogen and serum antibody to assess the progression of chronic periodontitis: a 24-mo prospective multicenter cohort study2016
Author(s)
Morozumi T, Nakagawa T, Nomura Y, Sugaya T, Kawanami M, Suzuki F, Takahashi K, Abe Y, Sato S, Makino-Oi A, Saito A, Takano S, Minabe M, Nakayama Y, Ogata Y, Kobayashi H, Izumi Y, Sugano N, …., Yoshimura A, Hara Y, ….
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Journal Title
Journal of Periodontal Research
Volume: 印刷中
Issue: 6
Pages: 768-778
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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