| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
The expression of the apoptosis inhibitor gene, FLIP mRNA, was examined from periodontitis gingiva by RT-PCR method, and it was possible to detect from approximately 40% of the samples. This result was consistent with the result that chronic inflammation persisted as a result of inhibition of cell death by apoptosis of lymphocytes infiltrating periodontitis gingiva. On the other hand, FLIP mRNA was not detected from the oral neutrophils, supporting the result that the expression level of pro-apoptotic Bad was high and the expression level of inhibitory phosphorylation was significantly low.
The increased levels of IL-1 beta, IL-6 and IL-17 in GCF were interpreted as a result of inflammation and immune response in periodontal tissues. Both necrosis and apoptosis may be involved in the mechanism because neutrophils in oxidative stress have decreased intracellular glutathione concentration and caspase 8 activity.
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