Antioxidant activity of curcumin: Porphyromonas gingivalis infection modifies oral microcirculation and aortic vascular function in the stroke-prone spontaneously hypertensive rat (SHRSP).

• Project/Area Number: 26463147
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Kanagawa Dental College
• Principal Investigator: Wada-Takahashi Satoko 神奈川歯科大学, 大学院歯学研究科, 准教授 (30301592)
• Co-Investigator(Kenkyū-buntansha): 浜田 信城 神奈川歯科大学, 大学院歯学研究科, 教授 (20247315) ; 松尾 雅斗 神奈川歯科大学, 大学院歯学研究科, 教授 (30190416) ; 高橋 俊介 神奈川歯科大学, 大学院歯学研究科, 教授 (60206810) ; 李 昌一 神奈川歯科大学, 歯学部, 教授 (60220795)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 歯周病 / 活性酸素種 / 高血圧 / 歯周病治療学 / 循環器疾患

Outline of Final Research Achievements

Periodontal disease is characterized by both inflammation and alveolar bone resorption. If patients predisposed to any systemic life-style related diseases are infected with P. gingivalis, periodontal tissue and other systemic vascular dysfunctions and failures may also slowly progress. Our results suggest that altered endothelial function may occur in gingival tissue in both periodontitis and stroke animal models. Oxidative stress induced by ROS may be related to the vascular effects in periodontitis and stroke models. Therefore, it is likely that altered vascular function in oral microcirculation could be caused by the interaction between ROS and NO in periodontitis, similar to what occurs in stroke. The ROS scavenging activities of curcumin was examined using electron spin resonance (ESR) technique in vitro. Curcumin had potent free radical scavenging activity and an antioxidative effect that inhibited the oxidative action of ROS.

Research Products

• [Journal Article] Novel antioxidative nanotherapeutics in a rat periodontitis model: Reactive oxygen species scavenging by redox injectable gel suppresses alveolar bone resorption. (2016)
  • Author(s): Saita M, Kaneko J, Sato T, Takahashi SS, Wada-Takahashi S, Kawanmata R, Sakurai T, Lee MC, Hamada N, Kimoto K, Nagasaki Y.
  • Journal Title: Biomaterials Volume: 76 Pages: 292-301
  • DOI: 10.1016/j.biomaterials.2015.10.077
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Microcirculation alterations in experimentally induced gingivitis in dogs. (2016)
  • Author(s): Matsuo M, Okudera T, Takahashi S-S, Wada-Takahashi S, Maeda S, Iimura A.
  • Journal Title: Anatomical Science International Volume: 1 Issue: 1 Pages: 1-6
  • DOI: 10.1007/s12565-015-0324-8
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protective effects of (6R)-5,6,7,8-tetrahydro-L-biopterin on local ischemia/reperfusion-induced suppression of reactive hyperemia in rat gingiva (2016)
  • Author(s): Tanaka Y, Toyama T, Wada-Takahashi S, Sasaki H, Miyamoto C, Maehata Y, Yoshino F, Yoshida A, Takahashi S-S, Watanabe K, Lee M-C, Todoki K, Hamada N
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 58 Issue: 1 Pages: 69-75
  • DOI: 10.3164/jcbn.15-69
  • NAID: 130005116629
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Porphyromonas gingivalis infection modifies oral microcirculation and aortic vascular function in the stroke-prone spontaneously hypertensive rat (SHRSP). (2016)
  • Author(s): Funaki S, Tokutomi F, Wada-Takahashi S, Yoshino F, Yoshida A, Maehata Y, Miyamoto C, Toyama T, Sato T, Hamada N, Lee M-C, Takahashi S-S
  • Journal Title: Microbial Pathogenesis Volume: 92 Pages: 36-42
  • DOI: 10.1016/j.micpath.2015.12.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] α-glucosyl hesperidin suppressed the exacerbation of 5-fluorouracil-induced oral mucositis in the hamster cheek pouch. (2016)
  • Author(s): Yoshino F, Yoshida A, Toyama T, Wada-Takahashi S, Takahashi S-S
  • Journal Title: Journal of Functional Foods Volume: 21 Pages: 223-231
  • DOI: 10.1016/j.jff.2015.12.008
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Foresight of physical development indicated by the National Health and Nutrition Survey in Japan: An approach in terms of biomedical sciences. (2015)
  • Author(s): Maehata Y, Miyamoto C, Tsukinoki K, Takahashi S, Yoshino F, Wada-Takahashi S, Yoshida A, Tanaka A, Adachi T, Igoshi N, Shiba T, Kishikawa N, Imazato T, Suzuki I, Ihara H, Shimomura J, Okabe H, Yanagisawa T, Hosho A, Maehata E
  • Journal Title: International Journal of Analytical Bio-Science Volume: 3 Pages: 63-72
  • Peer Reviewed
• [Journal Article] Blue light irradiation-induced oxidative stress in vivo via ROS generation in rat gingival tissue. (2015)
  • Author(s): Yoshida A, Shiotsu-Ogura Y, Wada-Takahashi S, Takahashi S-S, Toyama T, Yoshino F
  • Journal Title: Journal of Photochemistry and Photobiology B: Biology Volume: 151 Pages: 48-53
  • DOI: 10.1016/j.jphotobiol.2015.07.001
  • Peer Reviewed / Open Access
• [Journal Article] Contribution of nitrergic nerve in canine gingival reactive hyperemia (2015)
  • Author(s): Shimada S, Todoki K, Omori Y, Toyama T, Matsuo M, Wada-Takahashi S, Takahashi SS, Lee MC
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 56 Issue: 2 Pages: 98-104
  • DOI: 10.3164/jcbn.14-71
  • NAID: 130004879385
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access
• [Journal Article] Medical-grade collagen peptide in injectables provides antioxidant protection. (2015)
  • Author(s): Kobayashi K, Maehata Y, Okada Y, Kusubata M, Hattori S, Tanaka K, Miyamoto C, Yoshino F, Yoshida A, Tokutomi F, Wada-Takahashi S, Komatsu T, Otsuka T, Takahashi SS, Lee MC
  • Journal Title: Pharmaceutical Development and Technology Volume: 20(2) Pages: 219-226
  • Peer Reviewed / Open Access
• [Journal Article] Porphyromonas gingivalis-induced alveolar bone loss is accelerated in the stroke-prone spontaneously hypertensive rat. (2015)
  • Author(s): Tokutomi F, Wada-Takahashi S, Sugiyama S, Toyama T, Sato T, Hamada N, Tsukinoki K, Takahashi SS, Lee MC
  • Journal Title: Archives of Oral Biology Volume: 60(6) Pages: 911-918
  • Peer Reviewed / Open Access
• [Journal Article] Humoral response to therapeutic low-intensity pulsed ultrasound (LIPUS) treatment of rat maxillary socket after the removal of a molar tooth. (2015)
  • Author(s): Hidaka K, Miyamoto C, Wada-Takahashi S, Saita M, Kawata A, Kawamata R, Maehata Y, Minabe M, Takahashi SS, Mikuni-Takagaki Y
  • Journal Title: International Journal of Analytical Bio-Science Volume: 3(1) Pages: 17-24
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reactive oxygen species scavenging activity of Jixueteng evaluated by electron spin resonance (ESR) and photon emission. (2014)
  • Author(s): Toyama T, Wada-Takahashi S, Takamichi M, Watanabe K, Yoshida A, Yoshino F, Miyamoto C, Maehata Y, Sugiyama S, Takahashi SS, Todoki K, Lee MC, Hamada N
  • Journal Title: Natural Product Communications Volume: 9(12) Pages: 1755-1759
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Blue light from dental resin curing unit causes light-induced vasoconstriction in isolated rat aorta. (2014)
  • Author(s): Yoshida A, Iwata S, Iizuka J, Takahashi SS, Wada-Takahashi S, Miyamoto C, Maehata Y, Ogura Y, Lee MC, Yoshino F
  • Journal Title: Oral Health and Dental Management Volume: 13(4) Pages: 1147-1151
  • Peer Reviewed / Open Access
• [Journal Article] <b>Fasudil, a Rho kinase inhibitor, suppresses tumor growth by inducing CXCL14/BRAK in head and neck squamous cell car</b><b>cinoma </b> (2014)
  • Author(s): Miyamoto C., Maehata Y, Motohashi K, Ozawa S, Ikoma T, Hidaka K, Wada-Takahashi S, Yoshino F, Yoshida A, Kubota E, Hata R, Lee M.C
  • Journal Title: Biomedical Research Volume: 35 Issue: 6 Pages: 381-388
  • DOI: 10.2220/biomedres.35.381
  • NAID: 130004903867
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Therapeutic ultrasound prevents delayed healing of socket, possibly BRONJ, humorally. (2017)
  • Author(s): Hidaka K, Miyamoto C, Wada-Takahashi1 S, Kawamata R,Kawata A, Maehata Y, Saita M, Sato T, Watabe H, Tani-Ishii N, Takahashi S-S, Hamada N, Deguchi S, Mikuni-Takagaki Y.
  • Organizer: CED-IADR/NOF Oral Health Research Congress 2017
  • Int'l Joint Research
• [Presentation] マウス実験的歯周病炎モデルにおける生薬「鶏血藤」の循環改善効果 (2017)
  • Author(s): 遠山歳三，塗々木和男，高橋俊介，吉野文彦，吉田彩佳，松尾雅斗，高橋聡子，佐々木 悠，稲葉啓太郎，渡辺清子，浜田信城
  • Organizer: 日本歯周病学会 60 周年記念京都大会
• [Presentation] 低出力超音波パルス(LIPUS)刺激によりビスフォスフォネート関連顎骨壊死(BRONJ)を予防できるか―ステージ0を想定したモデルラットによる検討 (2017)
  • Author(s): 日髙恒輝，宮本千央，高橋聡子，川股亮太，河田亮，前畑洋次郎，斉田牧子，佐藤武則，渡部博隆，石井信之，高橋俊介，浜田信城，出口眞二，高垣裕子
  • Organizer: 日本歯周病学会60周年記念京都大会
• [Presentation] 加齢に伴う歯肉微小循環変化に対する物理的刺激 (歯肉マッサージ) の効果 (2017)
  • Author(s): 日髙弘一, 高橋聡子, 吉田彩佳，吉野文彦, 東 雅啓, 日髙恒輝, 松尾雅斗, 高橋俊介
  • Organizer: 神奈川歯科大学学会 第 52 回総会
• [Presentation] レーザードップラー血流計による咬合性外傷歯の歯肉血流計測 (2017)
  • Author(s): 小巻沙也佳, 杉山秀太, 尾崎博弥, 高橋聡子, 高橋俊介, 不島健持
  • Organizer: 神奈川歯科大学学会 第 52 回総会
• [Presentation] Alteration of oral vascular function in Porphyromonas gingivalis infection and/or stroke model. (2015)
  • Author(s): 舩木誠子，徳富文彬，高橋聡子，吉野文彦，吉田彩佳，前畑洋次郎，宮本千央，遠山歳三，佐藤武則，浜田信城，李 昌一，高橋俊介
  • Organizer: 神奈川歯科大学学会50周年記念総会
  • Place of Presentation: 横須賀
  • Year and Date: 2015-12-05
• [Presentation] Protective effects of (6R)-5,6,7,8-tetrahydro-L-biopterin on local ischemia/reperfusion-induced suppression of reactive hyperemia in rat gingiva. (2015)
  • Author(s): 田中優作，遠山歳三，高橋聡子，佐々木悠，宮本千央，前畑洋次郎，吉野文彦，吉田彩佳，高橋俊介，渡辺清子，李 昌一，塗々木和男，浜田信城
  • Organizer: 第 148 回 神奈川歯科大学学会例会
  • Place of Presentation: 横須賀
  • Year and Date: 2015-10-08
• [Presentation] 青色 LED 照射がラット口腔領域に及ぼす影響の検討 (2015)
  • Author(s): 吉田彩佳，小椋有香子，高橋俊介，高橋聡子，前畑洋次郎，宮本千央，堀 紀雄，遠山歳三，秦 光潤，前谷崇志，李 昌一，吉野文彦
  • Organizer: 第 15 回 日本抗加齢医学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2015-05-29
• [Presentation] Low-intensity Pulsed Ultrasound Prevents Healing of Infected Rat Extraction Socket (2015)
  • Author(s): Hidaka K, Miyamoto C, Kawamata R, Wada-Takahashi S, Takahashi SS, Maehata Y, Saita M, Sato T, Taniguchi M, Kawata A, Ozaki1 Y, Funayama Y, Watabe H, Tani-Ishii N, Hamada N, Deguchi S, Mikuni-Takagaki Y
  • Organizer: The International Association for Dental Research
  • Place of Presentation: Boston
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Inhibitory effect of Redox Injectable Gel on P.gingivalis-induced bone loss (2015)
  • Author(s): Saita M, Kaneko J, Sato T, Takahashi SS, Wada-Takahashi S, Kawamata R, Sakurai T, Lee MC, Hamada N, Nagasaki Y, Kimoto K
  • Organizer: The International Association for Dental Research
  • Place of Presentation: Boston
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] ラット抜歯窩に照射した低出力超音波パルスが全身的に及ぼす影響 Effect of Low-intensity Pulsed Ultrasound in Rats with Healing Socket (2015)
  • Author(s): 日髙恒輝，宮本千央，高橋聡子，斉田牧子，川股亮太，前畑洋次郎，三辺正人，高橋俊介，高垣裕子
  • Organizer: 第18回 超音波骨折治療研究会
  • Place of Presentation: 神戸
  • Year and Date: 2015-01-17
• [Presentation] ラット抜歯窩治癒に及ぼす低出力超音波パルス（LIPUS）の影響 (2015)
  • Author(s): 日髙恒輝, 宮本千央, 高橋聡子, 斉田牧子, 川股亮太, 前畑洋次郎, 三辺正人, 高橋俊介, 高垣裕子
  • Organizer: 第146回神奈川歯科大学学会例会
  • Place of Presentation: 横須賀
  • Year and Date: 2015-01-08
• [Presentation] Fasudil, a Rho kinase inhibitor, suppresses tumor growth by inducing CXCL14/BRAK in head and neck squamous cell carcinoma. (2014)
  • Author(s): 本橋一彦，宮本千央，前畑洋次郎，吉田彩佳，高橋聡子，高橋俊介，吉野文彦
  • Organizer: 第49回 神奈川歯科大学学会・総会
  • Place of Presentation: 横須賀
  • Year and Date: 2014-11-29
• [Presentation] Reactive oxygen species scavenging activity of Jixueteng evaluated by electron spin resonance (ESR) and photon emission (2014)
  • Author(s): 高道麻臣，遠山歳三，高橋聡子，吉田彩佳，吉野文彦，宮本千央，前畑洋次郎，渡辺清子，杉山秀太，塗々木和男，李 昌一，浜田信城，高橋俊介
  • Organizer: 第49回 神奈川歯科大学学会・総会
  • Place of Presentation: 横須賀
  • Year and Date: 2014-11-29
• [Presentation] ラット抜歯窩に照射した低出力超音波パルス (LIPUS) が全身に及ぼす影響 (2014)
  • Author(s): 日髙恒輝, 宮本千央, 高橋聡子, 斉田牧子, 川股亮太, 前畑洋次郎, 三辺正人, 高橋俊介, 高垣裕子
  • Organizer: 第49回神奈川歯科大学学会総会
  • Place of Presentation: 横須賀
  • Year and Date: 2014-11-29
• [Presentation] Development of Redox Therapy for Periodontal Disease by Redox Injectable Gel (2014)
  • Author(s): Saita M, Kaneko J, Sato T, Takahashi SS, Wada-Takahashi S, Kawamata R, Sakurai T, Lee MC, Hamada N, Kimoto K, Nagasaki Y
  • Organizer: IWAMSN
  • Place of Presentation: Vietnam
  • Year and Date: 2014-11-02 – 2014-11-04
  • Invited
• [Presentation] 抜歯窩に照射した低出力超音波パルスがラットに与える影響 (2014)
  • Author(s): 日髙恒輝, 高橋俊介, 高橋聡子, 根本賢治, 岩崎和人, 秋月達也, 高垣裕子, 三辺正人
  • Organizer: 第57回秋季日本歯周病学会学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2014-10-19
• [Presentation] ROCK 阻害剤 Fasudil の頭頚部扁平上皮癌における CXCL14/BRAK を介した抗腫瘍効果の検討 (2014)
  • Author(s): 宮本千央，前畑洋次郎，高橋聡子，吉野文彦，吉田彩佳，高橋俊介，李 昌一
  • Organizer: 第56回 歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] マウス実験的歯周炎モデルにおける生薬「鶏血藤」の循環改善効果 (2014)
  • Author(s): 遠山歳三，塗々木和男，高橋俊介，吉野文彦，吉田彩佳，松尾雅斗，高橋聡子，渡辺清子，浜田信城
  • Organizer: 第56回 歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] レドックスインジェクタブルゲルによる歯周病に対する抗酸化治療へのアプローチ (2014)
  • Author(s): 斉田牧子，金子純也，佐藤武則，高橋俊介，高橋聡子，川股亮太，櫻井 孝，浜田信城，李 昌一，木本克彦，長崎幸夫
  • Organizer: 日本酸化ストレス学術大会
  • Place of Presentation: 京都
  • Year and Date: 2014-09-04 – 2014-09-05
• [Presentation] Effect of Therapeutic Low-intensity Pulsed Ultrasound (LIPUS) after the Treatment of Rat Extraction Socket (2014)
  • Author(s): Hidaka K, Miyamoto C, Wada-Takahashi S, Kawamata R, Maehata Y, Minabe M, Takahashi SS, Mikuni-Takagaki Y
  • Organizer: FEBS EMBO 2014 Conference
  • Place of Presentation: Paris
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] ROCK Specific Inhibitor Fasudil Suppresses Head and Neck Squamous Cell Carcinoma Growth by Stimulating Gene Expression and Protein Secretion of the Chemokine CXCL14/BRAK (2014)
  • Author(s): Miyamoto C, Ozawa S, Ikoma T, Wada-Takahashi S, Takahashi SS, Yoshida A, Yoshino F, Hata RI, Lee MC, Maehata Y
  • Organizer: FEBS EMBO 2014 Conference
  • Place of Presentation: Paris
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] The novel collagen peptide reduced the photo-aging induced by UVA irradiation (2014)
  • Author(s): Maehata Y, Kobayashi K, Miyamoto C, Takahashi SS, Yoshino F, Wada-Takahashi S, Yoshida A, Lee MC
  • Organizer: FEBS EMBO 2014 Conference
  • Place of Presentation: Paris
  • Year and Date: 2014-08-30 – 2014-09-04
• [Presentation] レドックスインジェクタブルゲルを用いた抗酸化治療へのアプローチ (2014)
  • Author(s): 斉田牧子，金子純也，佐藤武則，高橋俊介，高橋聡子，川股亮太，櫻井 孝，浜田信城，李 昌一，木本克彦，長崎幸夫
  • Organizer: 日本 DDS 学会
  • Place of Presentation: 東京
  • Year and Date: 2014-07-30 – 2014-07-31
• [Presentation] Development of Redox Therapy for Periodontitis using Redox Injectable Gel (2014)
  • Author(s): Saita M, Kaneko J, Sato T, Takahashi SS, Wada-Takahashi S, Kawamata R, Sakurai T, Lee MC, Hamada N, Kimoto K, Nagasaki Y
  • Organizer: Controlled Released Society
  • Place of Presentation: Chicago
  • Year and Date: 2014-07-13 – 2014-07-16