Development of a genetic test for Streptococcus sobrinus

• Project/Area Number: 26463159
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Niigata University
• Principal Investigator: KANEKO Noboru 新潟大学, 医歯学系, 助教 (00397126)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 歯学 / ミュータンスレンサ球菌 / 齲蝕 / ソブリヌス菌 / グルカン

Outline of Final Research Achievements

Streptococcus sobrinus possesses a high ability to produce water-insoluble glucan (WIG), that contributes to accumulation on tooth surface and subsequent dental caries. This bacterium produces GtfI enzyme coded by gtfI gene that synthesizes WIG. We analyzed the association between WIG synthesis by clinical isolates of S. sobrinus and variation in the base sequence of gtfI gene. Relatively large numbers of missense mutations were observed in dextran-binding domain that is located in 3' end side of gtfI gene. We found that an isolate of S. sobrinus with one of the missense mutations in the dextran-binding domain showed particularly high WIG synthesis ability.

Research Products

• [Journal Article] Serum antibody to Porphyromonas gingivalis in metabolic syndrome among an older Japanese population (2016)
  • Author(s): Iwasaki M, Minagawa K, Sato M, Kaneko N, Imai S, Yoshihara A, Miyazaki H
  • Journal Title: Gerodontology Volume: 33 Pages: 193-200
  • Peer Reviewed
• [Journal Article] パパイン含有ゲル併用舌清掃による舌苔除去効果 (2016)
  • Author(s): 濃野 要，山賀孝之，金子 昇，宮﨑秀夫
  • Journal Title: 日本口腔衛生学会雑誌 Volume: 66 Pages: 9-14
  • NAID: 110010039436
  • Peer Reviewed
• [Journal Article] ホエイペプチド配合免疫調整流動食経口摂取が歯周炎患者における歯肉溝滲出液の炎症性サイトカインに与える影響 (2015)
  • Author(s): 久保田健彦，冨田尊志，濃野 要，阿部大輔，清水太郎，杉田典子，金子 昇，根津 新，川島昭浩，坪井 洋，佐々木 一，吉江弘正
  • Journal Title: 日本歯科保存学雑誌 Volume: 58 Pages: 109-116
  • NAID: 130005068919
  • Peer Reviewed
• [Journal Article] Serum antibody to Porphyromonas gingivalis in metabolic syndrome among an older Japanese population (2015)
  • Author(s): Iwasaki M, Minagawa K, Sato M, Kaneko N, Imai S, Yoshihara A, Miyazaki H
  • Journal Title: Gerodontology Volume: 32
  • Peer Reviewed
• [Presentation] Streptococcus sobrinus臨床分離株のgtfI遺伝子における多様性の検討 (2016)
  • Author(s): 金子 昇，濃野 要，山賀孝之，葭原明弘，花田信弘，宮﨑秀夫
  • Organizer: 第65回日本口腔衛生学会・総会
  • Place of Presentation: 東京医科歯科大学他（東京都文京区）
  • Year and Date: 2016-05-27
• [Presentation] 舌清掃におけるパパイン含有ゲル併用の有効性 (2015)
  • Author(s): 濃野 要，山賀孝之，金子 昇，宮﨑秀夫
  • Organizer: 第64回日本口腔衛生学会・総会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2015-05-27
• [Presentation] Streptococcus mutansのGtfB酵素C末端側におけるアミノ酸配列とう蝕との関連性 (2014)
  • Author(s): 金子 昇，濃野 要，葭原明弘，花田信弘，宮﨑秀夫
  • Organizer: 日本口腔衛生学会
  • Place of Presentation: 熊本市
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] 乳幼児健診に併設し歯科衛生士が実施する個別指導による歯科保健行動の変容 (2014)
  • Author(s): 皆川久美子，葭原明弘，金子昇，林 悠子，藤山友紀，宮﨑秀夫
  • Organizer: 日本口腔衛生学会
  • Place of Presentation: 熊本市
  • Year and Date: 2014-05-29 – 2014-05-31