Establishment of the novel oral health care guidelines based on oral microbiome analysis

Research Project

Project/Area Number	26463165
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Social dentistry
Research Institution	Kyushu University
Principal Investigator	Yamanaka Wataru 九州大学, 歯学研究院, 共同研究員 (20726228)
Co-Investigator(Kenkyū-buntansha)	柴田幸江九州大学, 歯学研究院, 助教 (30274476) 竹下徹九州大学, 歯学研究院, 准教授 (50546471)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	唾液 / 細菌叢 / 舌苔 / プラーク / 歯周病 / 次世代シーケンサー / マイクロバイオーム
Outline of Final Research Achievements	Saliva (SL), tongue coating (TC), supragingival plaque (AP), and subgingival plaque (BP) samples were collected twice from 14 patients with periodontitis who visited a dental clinic at their initial visit (pre-therapy samples) and after periodontal therapy (post-therapy samples). A principal coordinate analysis (PCoA) plot demonstrated that SL microbiota was distinct from both BP and AP microbiota, but closely similar to TC microbiota both pre- and post-therapy. Temporal variation following therapy in the SL and TC bacterial population was significantly smaller than in the AP microbiota, and relative abundances of the predominant bacterial species changed similarly in the SL and TC microbiota. These results suggested that the SL microbiota might originate from the TC microbiota.