Basic study on the effects of nutritional status on mucosal damage due to intratracheal aspiration

Research Project

Project/Area Number	26463212
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Fundamental nursing
Research Institution	Iwate Prefectural University
Principal Investigator	Hirano Akihiko 岩手県立大学, 看護学部, 准教授 (30305255)
Co-Investigator(Renkei-kenkyūsha)	TAKEDA Toshiaki 岩手県立大学, 看護学部, 教授 (40305248) KAWASAKI Masashi 岩手県立大学, 盛岡短期大学部, 准教授 (80299833)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000) Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	看護技術 / 気管内吸引 / 吸引圧 / 粘膜損傷 / 動物実験 / 細胞接着分子 / Ⅳ型コラーゲン / 栄養状態 / 栄養不良 / ウサギ / ELISA法 / ウサギ気管 / ELISA / ラミニン / フィブロネクチン / コラーゲンⅣ型 / ELISAkit / コラーゲンⅣ
Outline of Final Research Achievements	We aspirated the trachea of rabbits in undernutrition state under the assumption of tracheal aspiration of malnourished patients. Tracheal mucosal damage tended to occur in the undernutrition group than in the healthy group. Responsible factors were laminin, which adheres to epithelial cells and basal cells, weakening of adhesive force due to decrease in fibronectin adhering to the basement membrane and connective tissue, and weakening of the basement membrane due to decreased type IV collagen (constituent of the basement membrane supporting epithelial cell adhesion). We prepared rabbits in a state of undernutrition (rabbits that fasted while receiving only water and those that received reduced intake of food) and measured the quantity of three proteins contained in tracheal tissue. The quantity of all proteins in the undernutrition group was lower than that in the healthy group.