Novel mechanisms for unabosorbable food factors with anti-life style-related deseases activities

• Project/Area Number: 26500003
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrated Nutrition Science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: MIURA Yutaka 東京農工大学, (連合)農学研究科(研究院), 教授 (10219595)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: スフィンゴミエリン / 生活習慣病 / 腸管透過性 / エキソソーム / 難吸収性食品因子 / アスパラチン / 難吸収性 / 小腸上皮

Outline of Final Research Achievements

Some food factors are difficult to be absorbed from an intestinal tract, although they showed clear physiological functions. To elucidate the mode of actions of such unabsorbable food factors, the effect of sphingomyelin (SPM) on the intestinal permeability and serum exosomes was investigated. SPM is shown to increase the intercellular permeability of the intestinal epithelial cells, which may be one of the mechanisms of sphingomyelin functions. Serum exosomes could increase glucose uptake by L6 myotubes and dietary SPM augmented the serum exosome functions. These results suggest novel mode of actions of SPM via intestinal tracts and serum exosomes.

Research Products

• [Int'l Joint Research] Agricultural Research Council/Medical Research Council(南アフリカ)
• [Journal Article] Poly(A)-specific ribonuclease regulates the processing of small-subunit rRNAs in human cells. (2017)
  • Author(s): Ishikawa H., Yoshikawa H., Izumikawa K., Miura Y., Taoka M., Nobe Y., Yamauchi Y., Nakayama H., Simpson R. J., Isobe T. and Takahashi N..
  • Journal Title: Nucleic Acids Res. Volume: 45 Issue: 6 Pages: 3437-3447
  • DOI: 10.1093/nar/gkw1047
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Antidiabetic effect of enterolactone in cultured muscle cells and in type 2 diabetic model db/db mice. (2016)
  • Author(s): Zhou F., Furuhashi K, Son MJ, Toyozaki M, Yoshizawa F, Miura Y, and Yagasaki K.
  • Journal Title: Cytotechnology Volume: - Issue: 3 Pages: 493-502
  • DOI: 10.1007/s10616-016-9965-2
  • Peer Reviewed
• [Journal Article] Potential of rooibos, its major flavonoid C-glucosides and Z-2-(β-D-gluco- pyranoloxy)-3-phenylpropenoic acid in prevention of metabolic syndrome. (2016)
  • Author(s): Muller, C.J.F., Malherbe, C.J., Chellan, N., Yagasaki, K., Miura, Y., and Joubert, E.
  • Journal Title: Critical Reviews in Food Science and Nutrition Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Human nucleolar protein Nop52 (RRP1/NNP-1) is involved in site 2 cleavage in internal transcribed spacer 1 of pre-rRNAs at early stages of ribosome biogenesis. (2015)
  • Author(s): Yoshikawa H, Ishikawa H, Izumikawa K, Miura Y, Hayano T, Isobe T, Simpson RJ, Takahashi N.
  • Journal Title: Nucleic Acids Res. Volume: 43 Issue: 11 Pages: 5524-5536
  • DOI: 10.1093/nar/gkv470
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Antidiabetic effect of green rooibos (Aspalathus linearis) extract in cultured cells and type 2 diabetic model KK-Ay mice. (2014)
  • Author(s): R Kamakura, MJ Son, D De Beer, E Joubert, Y Miura, K Yagasaki
  • Journal Title: Cytotechnology Volume: - Issue: 4 Pages: 699-710
  • DOI: 10.1007/s10616-014-9816-y
  • Peer Reviewed
• [Presentation] 食品由来スフィンゴ脂質の抗がん・抗炎症作用 (2016)
  • Author(s): 三浦 豊
  • Organizer: 第70回日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学（兵庫県西宮市）
  • Year and Date: 2016-05-14
  • Invited
• [Presentation] 難吸収性食品因子スフィンゴミエリンの小腸上皮細胞の細胞間透過性への影響 (2016)
  • Author(s): 大川 澪、野村 義宏、三浦 豊
  • Organizer: 第70回日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学（兵庫県西宮市）
  • Year and Date: 2016-05-14
• [Presentation] 細胞膜糖脂質GM3によるマウスメラノーマ細胞運動能制御機構の解明 (2016)
  • Author(s): 三崎 公平、花房 知美、橋本 康太郎、及川 洋祐、三浦 豊
  • Organizer: 第70回日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学（兵庫県西宮市）
  • Year and Date: 2016-05-14
• [Presentation] 抗炎症作用を有するハニーブッシュ抽出物のスクリーニングとその作用の検証 (2016)
  • Author(s): 中溝 優、Elizabeth Joubert、Christo Muller、新井 浩司、野村 義宏、三浦 豊
  • Organizer: 第70回日本栄養・食糧学会大会
  • Place of Presentation: 武庫川女子大学（兵庫県西宮市）
  • Year and Date: 2016-05-14
• [Presentation] Induction of hepatoma cell death by egg-derived sphingomyelin and its molecular mechanism (2016)
  • Author(s): Kanako Ymamada, Kana Yamada, Natsuko Takamiya and Yutaka Miura
  • Organizer: The 29th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2016 Kobe)
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
• [Presentation] Screening of anti-diabetic food factors using cell cultures and animal models. (2015)
  • Author(s): Miura Y. and Yagasaki K.
  • Organizer: 2nd Joint International Symposium on Animal Cell Technology for Asian Network-by JAACT, TCELS and MU- “Biologics and Foods: Trend in Technology, Regulation and Safety”
  • Place of Presentation: Mahidol University, Thailand
  • Year and Date: 2015-11-18
  • Int'l Joint Research / Invited
• [Presentation] スフィンゴミエリンによる肝癌細胞死誘導とその機構解析 (2015)
  • Author(s): 山田佳奈、高宮奈津子、三浦 豊
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-26 – 2015-03-29