Molecular mechanism of stem cell-inducing reprogramming by epithelial mesenchymal transition
| Project/Area Number |
26501006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | Teikyo University of Science & Technology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
斉藤 史明 帝京大学, 医学部, 准教授 (40286993)
松村 喜一郎 帝京大学, 医学部, 教授 (50260922)
萩原 宏毅 帝京科学大学, 医療科学部, 教授 (80276732)
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| Research Collaborator |
Anura Rambukkana University of Edinburgh, MRC Centre for Regenerative Medicine, Professor
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | リプログラミング / ハンセン病原因菌 / 上皮間葉転換 / 幹細胞 / 間葉系幹細胞 / シュワン細胞 / 腫瘍幹細胞 / ハンセン病 / 人工的幹細胞誘導法 / 国際情報交換（英国） |
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| Outline of Final Research Achievements |
Snai1 was introduced into IMS32(immortalized mouse Schwann cell line) or adult mouse primary Schwann cells using lentivirus. In both Snai1-introduced Schwann cells, their morphologies changed to fibroblast-like appearance suggesting that epithelial mesenchymal transition (EMT) occurred. Also IMS32 acquired sphere-forming capacity, and differentiation potential to oil red O-positive preadipocyte-like cells, while differentiation potential to chondrocytes was not seen. On the other hand, Snai1 introduction did not induce these stem cell-like properties in adult mouse primary Schwann cells. These results will be molecular basis for regenerative therapy using these reprogrammed Schwann cell-derived stem cell-like cells.
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Report
(4 results)
Research Products
(4 results)
