Isolation of a novel human-mesenchymal stem cells based on the mechanism of Notch/ glycolytic pathway.
| Project/Area Number |
26501007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | Kindai University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MORIYAMA Mariko 近畿大学, 薬学総合研究所, 客員准教授 (40595295)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 間葉系幹細胞 / 分化能 / Notchシグナル / 解糖系 / 代謝 / 低酸素 |
|---|
| Outline of Final Research Achievements |
Human adipose tissue-derived multilineage progenitor cells (hADMPCs) are attractive for cell therapy and
tissue engineering because of their multipotency and ease of isolation without serial ethical issues. Here, we show that Notch signaling is required for glycolysis regulation under hypoxic conditions. Our results demonstrate that 5% O2 dramatically increased the glycolysis rate, improved the proliferation efficiency, prevented senescence, and maintained the multi potency of hADMPCs. Hypoxia significantly increased the level of activated Notch1 and expression of its downstream gene, HES1. Furthermore, Hypoxia markedly increased glucose consumption and lactate production, which decreased back to normoxic levels on treatment with a g-secretase inhibitor. We also found that HES1 was involved in induction of GLUT3, TPI, and PGK1 in addition to reduction of TIGAR and SCO2 expression. These results clearly suggest that Notch signaling regulates glycolysis under hypoxic conditions.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Beneficial Effects of the Genus Aloe on Wound Healing, Cell Proliferation, and Differentiation of Epidermal Keratinocytes.2016
Author(s)
Moriyama M, Moriyama H, Uda J, Kubo H, Nakajima Y, Goto A, Akaki J, Yoshida I, Matsuoka N, Hayakawa T.
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Journal Title
PLoS One
Volume: 11
Issue: 10
Pages: e0164799-e0164799
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] ヒト皮膚創傷治癒の効果2015
Author(s)
中島佑香,森山博由, 森山麻里子, 早川堯夫.
Organizer
第６５回日本薬学会近畿支部会
Place of Presentation
大阪大谷大学キャンパス,大阪
Year and Date
2015-10-17
Related Report
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[Presentation] ヒト皮膚創傷治癒の効果.2015
Author(s)
中島佑香,森山博由, 森山麻里子,早川堯夫.
Organizer
生命機能リトリート
Place of Presentation
同志社大学リトリートセンター,滋賀
Year and Date
2015-08-25
Related Report
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