| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Lens crystalline protein Crystallin has been diverted to existing proteins randomly during their evolution depending on individual species. In order to elucidate the origin protein of bullfrog specific ρ-crystallin, mRNA sequence of ρ-crystallin-like protein present in each tissue was analyzed and its characteristics were analyzed.
As a proteome analysis method to support crystallome, we will evaluate Py-Tag, a novel stable isotope labeling reagent that specifically labels the amino group of lysine residues, and proceed to application to peptides and proteins Basic data was collected. After that, I thought about developing it for application to crystallin analysis.
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