| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
At present, endoscopy relies almost exclusively on optical microscopy and the accurate analysis such as MS interrogation is performed ex-situ using biopsy. In this work, a novel probing system is developed to perform an in-situ and in-vivo endoscopic mass spectrometry using a moving string for the sampling and transportation of material. The sampling system consists of a moving cotton thread driven by motorized pulleys. When the target surface is touched by the sampling probe, the cotton thread “wipes” and transports the adhered sample to the ion source. Depending on the target analytes, desorption electrospray and atmospheric pressure chemical ionization sources are employed interchangeably for the desorption and ionization.The surface under analysis is not subjected to heat, organic solvents, high voltage or charged droplets. In-situ endoscopic MS of a living mouse and the surface analysis inside a volunteer subject’s mouth are successfully demonstrated.
|
