Development of a platform for proteome analysis of clinical FFPE samples
| Project/Area Number |
26505005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | プロテオミクス / FFPE / LC-MS / プロテオーム / HE染色 / 高感度LC/MS / リン酸化プロテオミクス / 高感度分析 |
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| Outline of Final Research Achievements |
This study provided a novel protein-extraction method and highly sensitive LC-MS system suitable for proteome profiling of minuscule FFPE tissue slices. This system can be utilized as a standard platform for FFPE proteome analysis. It was suggested that hematoxylin and eosin staining caused loss of proteins from FFPE tissues. Therefore, more detailed analysis is required for evaluating whether HE-stained FFPE tissue slices are suitable for proteome profiling.
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Report
(4 results)
Research Products
(16 results)
