Development and application of a steroidomics system using supercritical fluid extraction and separation technologies
| Project/Area Number |
26505007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Kyushu University |
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Principal Investigator |
Bamba Takeshi 九州大学, 生体防御医学研究所, 教授 (10432444)
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| Research Collaborator |
Fukusaki Eiichiro
IZUMI Yoshihiro
TAGUCHI Kaori
NAKAO Motonao
Udi Jumhawan
YAMASHITA Toshiyuki
NAKANO Yosuke
SHIMOMURA Iichiro
SHIOMI Masashi
FUKUDA Shinji
Shimadzu Co.
GL Sciences Inc.
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 超臨界流体 / 超臨界流体クロマトグラフィー / 超臨界流体抽出 / 質量分析 / SFC/MS / ステロイド / メタボロミクス / ステロイドミクス |
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| Outline of Final Research Achievements |
We successfully developed an analysis method for comprehensive profiling of steroid skeleton compounds, including their isomers and conjugates. The constructed high-resolution system uses supercritical fluid chromatography/mass spectrometry and an on-line supercritical fluid extraction system. Additionally, we performed steroidomic analyses using intestinal bacterial flora to evaluate the effectiveness of our method. We successfully obtained specific steroid profiles depending on the presence or absence of intestinal bacteria or periodontal disease bacteria, as well as determined the effects of various diets. We also analyzed various disease model animal and clinical samples, captured component variations specific to various diseases, and identified marker metabolites.
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Report
(4 results)
Research Products
(105 results)
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[Journal Article] Intracellular metabolite β-glucosylceramide is an endogenous Mincle ligand possessing immunostimulatory activity2017
Author(s)
Masahiro Nagata, Yoshihiro Izumi, Eri Ishikawa, Ryoko Kiyotake, Rieko Doi, Satoru Iwai, Zakaria Omahdi, Toshiyuki Yamaji, Tomofumi Miyamoto, Takeshi Bamba, Sho Yamasaki
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 114
Issue: 16
DOI
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Peer Reviewed / Open Access
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[Journal Article] Lipophagy maintains energy homeostasis in the kidney proximal tubule during prolonged starvation.2017
Author(s)
Minami, S., Yamamoto, T., Takabatake, Y., Takahashi, A., Namba, T., Matsuda, J., Kimura, T., Kaimori, J., Matsui, I., Takeda, H., Takahashi, M., Izumi, Y., Bamba, T., Matsusaka, T., Niimura, F., Isaka, Y.
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Journal Title
Autophagy
Volume: in press
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Increased Dynamics of Tricarboxylic Acid Cycle and Glutamate Synthesis in Obese Adipose Tissue: IN VIVO METABOLIC TURNOVER ANALYSIS.2017
Author(s)
Nagao H, Nishizawa H, Bamba T, Nakayama Y, Isozumi N, Nagamori S, Kanai Y, Tanaka Y, Kita S, Fukuda S, Funahashi T, Maeda N, Fukusaki E, Shimomura I.
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Journal Title
J Biol Chem.
Volume: 292
Issue: 11
Pages: 4469-4483
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] オミクス解析に基づく血中リポタンパク質脂質解析法の開発2017
Author(s)
竹田浩章, 和泉自泰, Thanai Paxton, 加藤紀子, 堀江真之介, 長瀬 勝敏, 小池智也, 塩見雅志, 松田史生, 福﨑英一郎, 馬場健史
Organizer
第7回 学際的脂質創生研究部会講演会
Place of Presentation
九州大学西新プラザ(福岡県福岡市)
Year and Date
2017-01-27
Related Report
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[Presentation] Steroid profiling method for rat serum and plasma by gas chromatography/tandem mass spectrometry with large volume injection2016
Author(s)
Udi Jumhawan, Toshiyuki Yamashita, Motonao Nakao, Kuniyo Sugitate, Takeshi Serino, Ryoichi Sasano, Yoshihiro Izumi, Takeshi Bamba
Organizer
64th ASMS Conference on Mass Spectrometry and Allied Topics
Place of Presentation
Henry B. Gonzalez Convention Center, San Antonio, Texas, USA
Year and Date
2016-06-05
Related Report
Int'l Joint Research
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[Presentation] Automatically extraction and analysis using on-line supercritical fluid extraction (SFE) / supercritical fluid chromatography (SFC) system2015
Author(s)
Uchikata, T., Terada, H., Funada, Y., Izumi, Y., Bamba, T.
Organizer
SFC 2015, 9th International Conference on Packed-Column SFC
Place of Presentation
Loews Philadelphia, Philadelphia, Pennsylvania, USA.
Year and Date
2015-07-22
Related Report
Int'l Joint Research
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[Book] Polar Lipids: Biology, Chemistry, and Technology2015
Author(s)
Yamada, T., Nagasawa, Y., Taguchi, K., Fukusaki, E., Bamba, T.
Total Pages
544
Publisher
AOCS Press
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