Study on DNA adductomics related to DNA damage and repair involving environmental microorganisms
Project/Area Number |
26505010
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
オミクス計測科学
|
Research Institution | Yokohama City University |
Principal Investigator |
Kanaly Robert 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (00448656)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | DNAアダクトミクス / 多環芳香族炭化水素 / Biotransformation / DNA adductomics |
Outline of Final Research Achievements |
Bacteria that biodegrade genotoxicants such as polycyclic aromatic hydrocarbons (PAHs) play important roles in carbon cycling of environmental pollutants. During PAH biodegradation, metabolite production may cause DNA damage and mutations which have implications for bacterial evolution. Biodegradation and exposure experiments were conducted with Sphingobium barthaii KK22. PAH-catechol metabolites were produced during biotransformation and gene expression related to PAH-catechol production was confirmed by RT-PCR. DNA adducts, including a PAH-catechol adduct were identified through DNA damage assessment by LC/ESI-MS/MS DNA adductomics and provided first direct evidence that bacteria are subjected to bulky DNA damage during PAH exposure. Increased levels of reactive oxygen species and oxidative DNA adducts were confirmed. RNA transcriptome analyses revealed that more than 600 genes were differentially expressed from PAH-catechol exposure including genes involved in DNA damage repair.
|
Report
(5 results)
Research Products
(21 results)
-
[Journal Article] Internalization of a novel, huge lectin from Ibacus novemdentatus (slipper lobster) induces apoptosis of mammalian cancer cells.2017
Author(s)
Fujii Y, Fujiwara T, Koide Y, Hasan I, Sugawara S, Rajia S, Kawsar SM, Yamamoto D, Araki D, Kanaly RA, Ogawa Y, Fujita H, Ozeki Y.
-
Journal Title
Glycoconj J
Volume: 34
Issue: 1
Pages: 85-94
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
[Journal Article] Analysis of the fine-tuning of cyanobacterial circadian phase by monochromatic light and long day conditions.2016
Author(s)
Kobayashi T, Obana Y, Kuboi N, Kitayama Y, Hayashi S, Oka M, Wada N, Arita K, Shimizu T, Sato M, Kanaly RA, Kutsuna S.
-
Journal Title
Plant Cell Physiol.
Volume: 57
Issue: 1
Pages: 105-114
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
[Journal Article] MytiLec, a mussel R-type lectin, interacts with surface glycan Gb3 on Burkitt’s lymphoma cells to trigger apoptosis through multiple pathways2015
Author(s)
Imtiaj Hasan, Shigeki Sugawara, Yuki Fujii, Yasuhiro Koide, Daiki Terada, Naoya Iimura, Toshiyuki Fujiwara, Keisuke G. Takahashi, Nobuhiko Kojima, Sultana Rajia, Sarkar M. A. Kawsar, Robert A. Kanaly, Hideho Uchiyama, Masahiro Hosono, Yukiko Ogawa, Hideaki Fujita, Jiharu Hamako, Taei Matsui, Yasuhiro Ozeki
-
Journal Title
Marine Drugs
Volume: 13
Issue: 12
Pages: 7377-7389
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
[Presentation] MytiLec, an R-type lectin from Mediterranean mussel regulates cancer cell growth through glycan-dependent signaling.2015
Author(s)
Hasan I, Fujii Y, Ogawa Y, Sugawara S, Hosono M, Koide Y, Rajia S, Kawsar S M A, Kanaly R A, Fujiwara T, Fujita H, Ozeki Y
Organizer
23rd International Glycoconjugates Symposium (GLYCO23)
Place of Presentation
Split, Croatia
Year and Date
2015-09-15
Related Report
Int'l Joint Research
-
-
[Book] Chapter 33, "Structural glycobiology for lectin to promote advanced biomedical research" Marine Glycobiology, Principles and Applications. (Edited by Se-Kwon Kim, ISBN: 9781498709613)2016
Author(s)
1. Hasan I, Fujii Y, Rajia S, Kawsar S M A, Sugawara S, Ogawa Y, Hosono M, Koide Y, Kanaly R A, Ozeki Y.
Publisher
Taylor & Francis Group, CRC press
Related Report
-
[Book] Chapter 21: SUEL/RBL-Lectins and their biomedical applications" Marine Omics, Principles and Applications (Se-Kwon Kim ed, ISBN 9781482258202)2016
Author(s)
5. Koide Y, Hasan I, Fujii Y, Kawsar SMA, Rajia S, Sugawara S, Ogawa Y, Hosono M, Kanaly RA, Ozeki Y.
Publisher
Taylor & Francis Group, CRC press
Related Report