| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Bacteria that biodegrade genotoxicants such as polycyclic aromatic hydrocarbons (PAHs) play important roles in carbon cycling of environmental pollutants. During PAH biodegradation, metabolite production may cause DNA damage and mutations which have implications for bacterial evolution. Biodegradation and exposure experiments were conducted with Sphingobium barthaii KK22. PAH-catechol metabolites were produced during biotransformation and gene expression related to PAH-catechol production was confirmed by RT-PCR. DNA adducts, including a PAH-catechol adduct were identified through DNA damage assessment by LC/ESI-MS/MS DNA adductomics and provided first direct evidence that bacteria are subjected to bulky DNA damage during PAH exposure. Increased levels of reactive oxygen species and oxidative DNA adducts were confirmed. RNA transcriptome analyses revealed that more than 600 genes were differentially expressed from PAH-catechol exposure including genes involved in DNA damage repair.
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