New Methods for Mass Spectrometry Imaging using SALDI and ESI Ionization
| Project/Area Number |
26505011
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Kansai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川崎 英也 関西大学, 化学生命工学部, 教授 (50322285)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 白金スパッタ蒸着 / レーザー脱離イオン化 / タッピング走査プローブ / エレクトロスプレー / イメージング質量分析 / 葉の農薬分布 / ナノピペット / フイルムの添加剤分布 / ナノピッペト / タッピング走査プローブエレクトロスプレー / ハイブリッド法 / グリセロ脂質 / ラット脳細胞 / 添加剤 |
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| Outline of Final Research Achievements |
The important requirements for mass spectrometry (MS) imaging include achieving desorption/ionization of target samples on a surface as locally as possible. Matrix-assisted laser desorption/ionization (MALDI) has been applied as a powerful ionization technique for MS imaging. However, since MALDI is operated under vacuum conditions, the sample preparation is less convenient for MS imaging. Recently, a number of ambient ionization methods such as desorption electrospray ionization (DESI) have been developed to allow MS imaging for the direct examination of unmodified samples. Here, we demonstrate new methods for MS imaging; one is platinum vapor deposition surface-assisted laser desorption/ionization (Pt-SALDI) and the other is tapping-mode scanning probe electrospray ionization (t-SPESI). These two methods were compared with the conventional methods of MALDI-MS and ambient ESI-MS to be evaluated in terms of simplicity of preparation and spatial resolution.
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Report
(4 results)
Research Products
(22 results)
