| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The important requirements for mass spectrometry (MS) imaging include achieving desorption/ionization of target samples on a surface as locally as possible. Matrix-assisted laser desorption/ionization (MALDI) has been applied as a powerful ionization technique for MS imaging. However, since MALDI is operated under vacuum conditions, the sample preparation is less convenient for MS imaging. Recently, a number of ambient ionization methods such as desorption electrospray ionization (DESI) have been developed to allow MS imaging for the direct examination of unmodified samples. Here, we demonstrate new methods for MS imaging; one is platinum vapor deposition surface-assisted laser desorption/ionization (Pt-SALDI) and the other is tapping-mode scanning probe electrospray ionization (t-SPESI). These two methods were compared with the conventional methods of MALDI-MS and ambient ESI-MS to be evaluated in terms of simplicity of preparation and spatial resolution.
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