| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Recently, we reported the first ribosomal peptide synthetic pathway in filamentous fungi, which is the ustiloxin one in Aspergillus flavus. Here, we further surveyed 14 Aspergillus strains for the ustiloxin type ribosomal peptide synthetic (ust-RiPS) pathways and compounds, using genome sequence analyses and LC-MS metabolite analyses. Two novel cyclic peptides synthesised by novel ust-RiPS pathways were identified, which are both composed of the amino acids in the core peptide sequences and cyclised by the ether bonds directly to aromatic rings, in the same manner with ustiloxin. However, the amino acid components and the structures are completely different among the three compounds. The genome information survey indicates that the ust-RiPS pathways are widely conserved in fungi, and there is a wide variety of the core peptide sequences. The results in this study strongly imply that the ust-RiPS pathways are a diverse and abundant resource of biosynthetic genes for cyclic peptides.
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