Rational investigation of fungal ribosomal peptide biosynthetic pathways
| Project/Area Number |
26505015
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Umemura Maiko 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 研究グループ付 (00552259)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 糸状菌 / 二次代謝経路 / リボソームペプチド |
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| Outline of Final Research Achievements |
Recently, we reported the first ribosomal peptide synthetic pathway in filamentous fungi, which is the ustiloxin one in Aspergillus flavus. Here, we further surveyed 14 Aspergillus strains for the ustiloxin type ribosomal peptide synthetic (ust-RiPS) pathways and compounds, using genome sequence analyses and LC-MS metabolite analyses. Two novel cyclic peptides synthesised by novel ust-RiPS pathways were identified, which are both composed of the amino acids in the core peptide sequences and cyclised by the ether bonds directly to aromatic rings, in the same manner with ustiloxin. However, the amino acid components and the structures are completely different among the three compounds. The genome information survey indicates that the ust-RiPS pathways are widely conserved in fungi, and there is a wide variety of the core peptide sequences. The results in this study strongly imply that the ust-RiPS pathways are a diverse and abundant resource of biosynthetic genes for cyclic peptides.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi.2016
Author(s)
Y. Ye, A. Minami, Y. Igarashi, M. Izumikawa, M. Umemura, N. Nagano, M. Machida, T. Kawahara, K. Shin-ya, K. Gomi, H. Oikawa
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Journal Title
Angewandte Chemie-International Edition
Volume: 55
Issue: 28
Pages: 8072-8075
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Class of cyclic ribosomal peptide synthetic genes in filamentous fungi2016
Author(s)
N. Nagano, M. Umemura (CA), M. Izumikawa, J. Kawano, T. Ishii, M. Kikuchi, K. Tomii, T. Kumagai, A. Yoshimi, M. Machida, K. Abe, K. Shin-ya, and K. Asai
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Journal Title
Fungal Genetics and Biology
Volume: 86
Pages: 58-70
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Ribosomal peptide biosynthetic pathways widely conserved in filamentous fungi2016
Author(s)
M. Umemura, N. Nagano, A. Yoshimi, Y. Ye, A. Minami, K. Abe, K. Shin-ya, H. Oikawa, M. Machida
Organizer
2016 SIMB Annual Meeting and Exhibition
Place of Presentation
Sheraton Hotel, New Orleans, US
Year and Date
2016-07-24
Related Report
Int'l Joint Research / Invited
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