Establishment of molecular basis for skeletal development in medaka
| Project/Area Number |
26506005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
宇宙生命学
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Inohaya Keiji 東京工業大学, 生命理工学院, 助教 (70302958)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 発生・分化 / 骨形成 / 骨芽細胞 / メダカ |
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| Outline of Final Research Achievements |
sp7/osterix is a zinc finger transcription factor that is essential for osteoblast differentiation in mammals. To verify the characteristic features of osteoblast-lineage cells in teleosts, medaka sp7 mutants were established using a transcription activator-like effector nuclease (TALEN) genome editing system. These mutants showed severe defects in the formation of skeletal structures, indicating that sp7/osterix gene is essential for skeletal development in medaka. In addition, the present study revealed that type X collagen a1 a (col10a1a)-positive cells are osteoblast-like cells and sp7/osterix gene expression in osteoblast-lineage cells is required for differentiation into mature osteoblasts to form the skeletal structures.
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Report
(5 results)
Research Products
(8 results)
