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Establishment of molecular basis for skeletal development in medaka

Research Project

Project/Area Number 26506005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field 宇宙生命学
Research InstitutionTokyo Institute of Technology

Principal Investigator

Inohaya Keiji  東京工業大学, 生命理工学院, 助教 (70302958)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords発生・分化 / 骨形成 / 骨芽細胞 / メダカ
Outline of Final Research Achievements

sp7/osterix is a zinc finger transcription factor that is essential for osteoblast differentiation in mammals. To verify the characteristic features of osteoblast-lineage cells in teleosts, medaka sp7 mutants were established using a transcription activator-like effector nuclease (TALEN) genome editing system. These mutants showed severe defects in the formation of skeletal structures, indicating that sp7/osterix gene is essential for skeletal development in medaka. In addition, the present study revealed that type X collagen a1 a (col10a1a)-positive cells are osteoblast-like cells and sp7/osterix gene expression in osteoblast-lineage cells is required for differentiation into mature osteoblasts to form the skeletal structures.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (4 results)

  • [Journal Article] The sp7 gene is required for maturation of osteoblast-lineage cells in medaka (Oryzias latipes) vertebral column development2017

    • Author(s)
      Azetsu Y, Inohaya K, Takano Y, Kinoshita M, Tasaki M, Kudo A.
    • Journal Title

      Dev Biol

      Volume: 431(2) Issue: 2 Pages: 252-262

    • DOI

      10.1016/j.ydbio.2017.09.010

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reiterative expression of pax1 directs pharyngeal pouch segmentation in medaka (Oryzias latipes)2016

    • Author(s)
      Okada K, Inohaya K, Mise T, Kudo A, Takada S, Wada H.
    • Journal Title

      Development

      Volume: in press

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] TGFβ-2 signaling is essential for osteoblast migration and differentiation during fracture healing in medaka fish2016

    • Author(s)
      Takeyama K, Chatani M, Inohaya K, Kudo A.
    • Journal Title

      Bone

      Volume: 86 Pages: 68-78

    • DOI

      10.1016/j.bone.2016.03.001

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Osteoblast and osteoclast behaviors in the turnover of attachment bones during medaka tooth replacement2016

    • Author(s)
      Mantoku A, Chatani M, Aono K, Inohaya K, Kudo A.
    • Journal Title

      Developmental Biology

      Volume: 409 Issue: 2 Pages: 370-381

    • DOI

      10.1016/j.ydbio.2015.12.002

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] メダカ脊椎形成における体節および硬節細胞の役割2017

    • Author(s)
      猪早敬二
    • Organizer
      日本動物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] メダカ脊椎形成に硬節細胞は関与するか?2017

    • Author(s)
      猪早敬二、工藤明
    • Organizer
      日本動物学会関東支部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] メダカ脊椎形成における体節の役割2016

    • Author(s)
      猪早敬二
    • Organizer
      日本動物学会関東支部 第68回大会
    • Place of Presentation
      神奈川大学・横浜キャンパス
    • Year and Date
      2016-03-12
    • Related Report
      2015 Research-status Report
  • [Presentation] 脊椎の分節パターニングに異常を示すメダカ変異体の解析2014

    • Author(s)
      福島慶子
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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