The elucidation of the life support mechanism under high vacuum condition: the application to space environment
Project/Area Number |
26506008
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
宇宙生命学
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Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
OHTA ISAO 浜松医科大学, 光尖端医学教育研究センター, 技術専門員 (20464133)
|
Co-Investigator(Kenkyū-buntansha) |
針山 孝彦 浜松医科大学, 医学部, 教授 (30165039)
高久 康春 浜松医科大学, 医学部, 特任助教 (60378700)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
|
Keywords | 走査電子顕微鏡 / NanoSuit / 高真空 / 生命維持 / バリアー機能 / FE-SEM / バリアー性能 |
Outline of Final Research Achievements |
In this research, with the aim of elucidating the life support mechanism in space, we have examined various living biological specimens protected with a polymerized thin film "NanoSuit". We used the high vacuum conditions (10-3-10-7 Pa) in the observation chamber of a field-emission scanning electron microscopy (FE-SEM) as the experimental space, and revealed that a simple surface modification by electron beams can equip some multicellular organisms with a strong tolerance against the high vacuum. Furthermore, to protect living single cells, we have developed a new solution “Surface Shield Enhancer (SSE)” that consists of glycerin and electrolytes as a component for NanoSuit. The SSE based NanoSuit affords a much stronger barrier to gas and/or liquid loss than the previous NanoSuit did and, since it allows more detailed images, it could significantly help to elucidate the ‘real’ organization of cells and their functions under the space environment.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] A modified ‘NanoSuit’ preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopy.2017
Author(s)
Takaku Y, Suzuki H, Kawasaki H, Ohta I, Ishii D, Hirakawa S, Tsutsui T, Matsumoto H, Takehara S, Nakane C, Sakaida K, Suzuki C, Muranaka Y, Kikuchi H, Konno H, Shimomura M, Hariyama T
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Journal Title
R Soc Open Sci
Volume: なし
Issue: 3
Pages: 160887-160887
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry.2015
Author(s)
Hameed S, Ikegami K, Sugiyama E, Matsushita S, Kimura Y, Hayasaka T, Sugiura Y, Masaki N, Waki M, Ohta I, Hossen MA, Setou M
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Journal Title
Anal Bioanal Chem
Volume: 407
Issue: 25
Pages: 7589-7602
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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-
-
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[Presentation] Dressing living organisms in a thin polymer membrane, the NanoSuit, for high-vacuum FE-SEM observation.2014
Author(s)
Hariyama T, Ohta I, Hirakawa S, Kawasaki H, Suzuki H, Ishii D, Muranaka Y, Shimomura M, Takaku Y
Organizer
日本顕微鏡学会
Place of Presentation
幕張メッセ国際会議場
Year and Date
2014-05-13
Related Report
Invited