Initial step of gravity sensing in cultured animal cells
| Project/Area Number |
26506009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
宇宙生命学
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 宇宙生命科学 / 微小重力 / 動物細胞 / 細胞力覚 |
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| Outline of Final Research Achievements |
Recent studies have cleared that individual cells can sense microgravity. However, this sensing mechanism remains largely unknown. We hypothesize that in response to altered gravity, the tension generated in SFs are altered, and that it cause gravity-dependent cellular responses. To verify it, we examined the substrate rigidity-dependent responses of mesenchymal stem cells (MSCs) under simulated microgravity. It is known that MSCs could change rigidity-dependently the tension in SFs to induce the rigidity-dependent responses. Actually at 1 g MSCs cultured on the soft substrate which could weaken the SF tension, decreased osteoblastic differentiation compared with those on the stiff substrate. However, the rigidity-dependent responses of MSCs were not found under simulated microgravity. These results suggest that stimulated microgravity could weaken the SF tension of MSCs even on the stiff substrate and that changes in the SF tension may mediate the gravity sensing of MSCs.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Identification of Meflin as a Potential Marker for Mesenchymal Stromal Cells2016
Author(s)
Maeda K, Enomoto A, Hara A, Asai N, Kobayashi T, Horinouchi A, Maruyama S, Ishikawa Y, Nishiyama T, Kiyoi H, Kato T, Ando K, Weng L, Mii S, Asai M, Mizutani Y, Watanabe O, Hirooka Y, Goto H, Takahashi M
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 22288-22288
DOI
Related Report
Peer Reviewed / Open Access
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