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The analysis of auxin function in reconstruction of cell wall polysaccharide by hyper gravity.

Research Project

Project/Area Number 26506015
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field 宇宙生命学
Research InstitutionThe University of Tokyo

Principal Investigator

Suzuki Masashi  東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (30342801)

Co-Investigator(Kenkyū-buntansha) 嶋田 幸久  横浜市立大学, 木原生物学研究所, 教授 (30300875)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsオーキシン / 細胞壁 / 重力 / シロイヌナズナ / 支持領域 / 伸長領域
Outline of Final Research Achievements

The elongation of floral stem of Arabidopsis was inhibited by hyper-gravity. The expression of Aux/IAA19, an auxin responsive gene, was also inhibited by hyper-gravity. Auxin is known to loosen cell wall. From these results, we speculated that hyper-gravity down regulates auxin and then the elongation of floral stem of Arabidopsis was inhibited by down regulated auxin signal. However, molecular sizes of xyloglucan were not different between wild type and auxin accumulated mutant. To understand the relationship between auxin and reconstruction of cell wall polysaccharide and cell elongation on gravity resistance, more detailed analyses have to be performed.
On the other hands, the expression of some genes encoding cytoskeletal proteins, such as myosins, was affected by hyper-gravity. We consider that the total analyses of gravity resistance system including cytoskeleton is important.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015 2014

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis2017

    • Author(s)
      Takato, Shin; KAKEI, Yusuke; Mitsui, Marie; Ishida, Yosuke; Suzuki, Masashi; Yamazaki, Chiaki; HAYASHI, Kenichiro; Ishii, Takahiro; Nakamura, Ayako; Soeno, Kazuo; SHIMADA, Yukihisa
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 印刷中 Issue: 7 Pages: 1320-1326

    • DOI

      10.1080/09168451.2017.1313694

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Transcriptional feedback regulation of YUCCA genes in response to auxin levels in Arabidopsis2015

    • Author(s)
      Masashi Suzuki, Chiaki Yamazaki, Marie Mitsui, Yusuke Kakei, Yuka Mitani, Ayako Nakamura, Takahiro Ishii, Kazuo Soeno, Yukihisa Shimada
    • Journal Title

      Plant Cell Rep

      Volume: TBD Issue: 8 Pages: 1343-52

    • DOI

      10.1007/s00299-015-1791-z

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Small-molecule auxin inhibitors that target YUCCA are powerful tools for studying auxin function.2015

    • Author(s)
      Kakei Y, Yamazaki C, Suzuki M, Nakamura A, Sato A, Ishida Y, Kikuchi R, Higashi S, Kokudo Y, Ishii T, Soeno K, Shimada Y
    • Journal Title

      Plant J

      Volume: 84 Issue: 4 Pages: 827-37

    • DOI

      10.1111/tpj.13032

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Involvement of auxin and brassinosteroid in gravity resistance of Arabidopsis inflorescence stem treated with hypergravity2017

    • Author(s)
      石山和、筧雄介、山崎千秋、鈴木優志、木村あかり、曽我康一、保尊隆亨、嶋田幸久
    • Organizer
      第58回日本植物生理学会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新奇エチレン様活性物質のStriga hermonthica種子発芽誘導活性2016

    • Author(s)
      水野翼、北畑信隆、鈴木優志、浅見忠男
    • Organizer
      植物化学調節学会第51回大会
    • Place of Presentation
      高知大学(高知県南国市)
    • Year and Date
      2016-10-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] シロイヌナズナの抗重力飯能におけるブラシノステロイドとオーキシンの機能解析2016

    • Author(s)
      石山和、筧雄介、山崎千秋、鈴木優志、嶋田幸久
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of compounds showing ethylene like activity2016

    • Author(s)
      Suzuki M, Kengoyama Y, Mizuno T, Imamura Y, Kitahata N, Abe K, Okada S, Asami T
    • Organizer
      22nd International Conference on Plant Growth Substances
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2016-06-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Feedback regulation of YUCCA gene expression in Auxin biosynthesis through SCFTIR1/AFB complex2016

    • Author(s)
      Takato S, Sato A, Suzuki M, Kakei Y, Hayashi K, Nakamura A, Soeno K, Shimada Y
    • Organizer
      22nd International Conference on Plant Growth Substances
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2016-06-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SCFTIR1複合体を介したオーキシン生合成のフィードバック制御機構2016

    • Author(s)
      高藤晋、三井麻理江、石田遙介、鈴木優志、筧雄介、山崎千秋、石井貴広、林謙一郎、藤岡正三、中村郁子、持田恵一、添野和雄、嶋田幸久
    • Organizer
      第57回日本植物生理学会
    • Place of Presentation
      岩手大学(岩手県・盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 筧雄介、山崎千秋、鈴木優志、中村郁子、佐藤明子、石田遙介、菊池理絵、東昌市、國土祐未子、石井貴広、添野和雄、嶋田幸久2016

    • Author(s)
      YUCCAを標的としたオーキシン生合成阻害剤~作用機構解析の続報
    • Organizer
      第57回日本植物生理学会
    • Place of Presentation
      岩手大学(岩手県・盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 5-methyl-3-phenylisoxazole-4-carboxylic acidはオーキシンシグナルを遮断する2015

    • Author(s)
      鈴木優志、宮崎翔、白井郁也、浅見忠男
    • Organizer
      植物化学調節学会第50回大会
    • Place of Presentation
      東京大学(東京都・文京区)
    • Year and Date
      2015-10-23
    • Related Report
      2015 Research-status Report
  • [Presentation] ブラシノステロイドによる細胞壁強度の制御はシロイヌナズナ花茎の伸長・重力応答領域を調節する2015

    • Author(s)
      筧雄介、山崎千秋、鈴木優志、曽我康一、保尊隆亨、嶋田幸久
    • Organizer
      植物化学調節学会第50回大会
    • Place of Presentation
      東京大学(東京都・文京区)
    • Year and Date
      2015-10-23
    • Related Report
      2015 Research-status Report
  • [Presentation] SCFTIR1/AFB complexを介したオーキシン生合成のフィードバック制御機構2015

    • Author(s)
      高藤晋、三井麻理江、石田遙介、鈴木優志、筧雄介、山崎千秋、石井貴広、林謙一郎、藤岡正三、中村郁子、持田恵一、添野和雄、嶋田幸久
    • Organizer
      植物化学調節学会第50回大会
    • Place of Presentation
      東京大学(東京都・文京区)
    • Year and Date
      2015-10-23
    • Related Report
      2015 Research-status Report
  • [Presentation] ブラシノステロイドによるシロイヌナズナ花茎の伸長・重力応答領域の決定分子機構の探索2015

    • Author(s)
      筧雄介、木村あかり、曽我康一、保尊隆亨、山崎千秋、鈴木優志、嶋田幸久
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 抗重力形態形成に着目したシロイヌナズナ花茎先端-基部軸の遺伝子発現二次元分布の網羅的解析2014

    • Author(s)
      筧雄介、山崎千秋、鈴木優志、嶋田幸久
    • Organizer
      日本宇宙生物科学会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2014-09-22 – 2014-09-23
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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