Mechanisms regulating transcriptional activation and repression by gravitropic response in root and cotyledon
Project/Area Number |
26506016
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
宇宙生命学
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Research Institution | Yokohama City University |
Principal Investigator |
Kakei Yusuke 横浜市立大学, 木原生物学研究所, 特任助教 (50636727)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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Keywords | 重力屈性 / オーキシン / シロイヌナズナ / ブラシノステロイド / トランスクリプトーム / 重力 |
Outline of Final Research Achievements |
Plant gravitropism is regulated by polar transport of auxin to the same direction as gravity. The subsequent molecular mechanism of the differential growth still remains to be elucidated. An auxin biomarker protein containing modified TIR1, DII region of Aux/IAA and FRET regions was developed to compare auxin concentration in shoot and root gravitropism. However, overexpression of the biomarker did not work well because of cell death. Next, we analyzed transcriptional changes caused by wide range of auxin concentrations. Inhibitors of auxin biosynthesis BBo and PPBo that target auxin synthase YUCCA were developed to decrease auxin concentration effectively. Arabidopsis and Mung bean were treated by gravity stimuli, several kinds of auxin and inhibitors. Interestingly, transcriptional auxin-responses after gravity stimuli were observed at non-bending tissue in addition to bending tissues. This result showed that auxin concentration is not the only regulator of differential growth.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Small-molecule auxin inhibitors that target YUCCA are powerful tools for studying auxin function.2015
Author(s)
Kakei Y, Yamazaki C, Suzuki M, Nakamura A, Sato A, Ishida Y, Kikuchi R, Higashi S, Kokudo Y, Ishii T, Soeno K, Shimada Y
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Journal Title
Plant J
Volume: 84
Issue: 4
Pages: 827-37
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Phenylboronic acid analogs are effective auxin biosynthesis inhibitors targeting YUCCA2016
Author(s)
Yusuke Kakei, Chiaki Yamazaki, Masashi Suzuki, Ayako Nakamura, Akiko Sato, Yosuke Ishida, Rie Kikuchi, Shouichi Higashi, Yumiko Yamasaki-Kokudo, Takahiro Ishii, Kazuo Soeno, Yukihisa Shimada
Organizer
第57回日本植物生理学会年会
Place of Presentation
盛岡
Year and Date
2016-03-18
Related Report
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[Presentation] Auxin biosynthesis inhibitor effectively targets YUCCA2015
Author(s)
Yusuke Kakei, Chiaki Yamazaki, Masashi Suzuki, Ayako Nakamura, Akiko Sato, Yosuke Ishida, Rie Kikuchi, Shouichi Higashi, Yumiko Yamasaki-Kokudo, Takahiro Ishii, Kazuo Soeno, Yukihisa Shimada
Organizer
Pacificjem 2015
Place of Presentation
Honolulu
Year and Date
2015-12-15
Related Report
Int'l Joint Research
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