| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Outline of Final Research Achievements |
Plant gravitropism is regulated by polar transport of auxin to the same direction as gravity. The subsequent molecular mechanism of the differential growth still remains to be elucidated. An auxin biomarker protein containing modified TIR1, DII region of Aux/IAA and FRET regions was developed to compare auxin concentration in shoot and root gravitropism. However, overexpression of the biomarker did not work well because of cell death. Next, we analyzed transcriptional changes caused by wide range of auxin concentrations. Inhibitors of auxin biosynthesis BBo and PPBo that target auxin synthase YUCCA were developed to decrease auxin concentration effectively. Arabidopsis and Mung bean were treated by gravity stimuli, several kinds of auxin and inhibitors. Interestingly, transcriptional auxin-responses after gravity stimuli were observed at non-bending tissue in addition to bending tissues. This result showed that auxin concentration is not the only regulator of differential growth.
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