Structural ans functional analysis of single-stranded DNA-binding protein DdrA

• Project/Area Number: 26506030
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 宇宙生命学
• Research Institution: Japan Aerospace EXploration Agency
• Principal Investigator: YAMADA Mitsugu 国立研究開発法人宇宙航空研究開発機構, 有人宇宙技術部門, 主任研究開発員 (80510924)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: タンパク質結晶解析 / 構造生物学 / 構造生物 / DNA修復

Outline of Final Research Achievements

In this study, crystallizaion experiments were cariied out to obtain the information of structural and functional relationship of DdrA, a DNA-related protein repair-related protein of Deinococcus radiodurans.
We have constructed expression vector of DdrA derived from multiple species and successfully selected the constructs suitable for crystallization. We also found that there is novel processing mechanism.
We are going to clarify the new processing mechanism as well as advance the crystal analysis in the future.