Sleep-Dependent Synaptic Plasticity Underlying Maintenance of Neuronal Circuits in the Brain.
| Project/Area Number |
26507001
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
睡眠科学
|
|---|
| Research Institution | Tohoku Institute of Technology (2015-2017)
Tohoku University (2014) |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
NAKAMURA Aritaka
ANZAI Yuka
Tsukada Ryo
MASUDA Yukiteru
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | シナプス可塑性 / AMPA 受容体 / 睡眠-覚醒 / シナプスホメオスタシス / 睡眠 / シナプス結合 / AMPA受容体 / パッチクランプ / ホメオスタシス / 感覚応答 / 海馬 / 大脳皮質 / 神経回路 / ノンレム睡眠 / 断眠 / 脳波解析 / 覚醒 |
|---|
| Outline of Final Research Achievements |
In this study, we investigated the sleep-wake stage dependent synaptic plasticity in the cortex and hippocampus and underlying mechanism of the plasticity. We made whole-cell patch-clamp recordings from pyramidal neurons of the somatosensory cortex and hippocampus in acute slices and recorded evoked EPSCs. We focused on trafficking of Ca-permeable AMPA (CP-AMPA) receptor, because presence of the receptor indicate the synapse was potentiated just before preparing slices. In this study, we tested whether CP-AMPA receptor antagonist affected AMPA receptor mediated the EPSCs. We found that the antagonist caused a significant reduction of EPSC amplitudes in awake rats, and did not have any significant effect on the amplitude in sleeping rats. These results indicate that CP-AMPA receptors are inserted into the synapse only during wakefulness both in the cortex and hippocampus. These two different brain regions are suggested to share the same mechanism of stage dependent synaptic plasticity.
|
Report
(5 results)
Research Products
(49 results)
