Analysis of the function of calcineurin in Drosophila sleep homeostasis
| Project/Area Number |
26507008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
睡眠科学
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| Research Institution | Nagoya City University |
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Principal Investigator |
Tomita Jun 名古屋市立大学, 大学院薬学研究科, 講師 (40432231)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ショウジョウバエ / 睡眠 / カルシニューリン / NMDA受容体 / アミノ酸トランスポーター / プロリン / 中心複合体 / モザイク解析法 / ドーパミン / 前大脳橋 / MARCM法 |
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| Outline of Final Research Achievements |
We found pan-neuronal knockdown of one of putative transporter family genes in Drosophila, whose mammalian homolog is a transporter for neutral amino acids such as proline and leucine, significantly decreased sleep. Interestingly, mutant flies with elevated levels of free proline in neurons significantly increased sleep. Our present working hypothesis is that proline activates NMDA receptor signaling and promotes sleep.
In order to identify a novel neural circuit that control Drosophila sleep, we focused on central complex neurons. We found that activation of neurons labeled by a Gal4 driver that expresses in the protocerebral bridge (PB) neurons, significantly decreased sleep. Using mosaic analysis with a repressible cell marker (MARCM), we found that activation of neurons projecting from the PB to the fan-shaped body (FB) and the noduli (NO) in the driver reduced sleep. These results suggest that specific small group of PB neurons are implicated in sleep regulation.
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Report
(4 results)
Research Products
(11 results)
