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Molecular mechanism of the floral initiation by florigen and its application to breeding

Research Project

Project/Area Number 26520307
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Food Cycle Research
Research InstitutionKyoto University

Principal Investigator

Ohki Izuru  京都大学, 工学研究科, 特定研究員 (80418574)

Project Period (FY) 2014-07-18 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsX線結晶解析 / 植物 / 植物ホルモン / 花成 / フロリゲン / 立体構造解析 / 植物花成 / 育種 / 構造生物学
Outline of Final Research Achievements

In higher plants, florigen is a mobile hormone that determines the flowering time, but its regulatory mechanism of flowering remains unclear. In this study, we focused on an anti-florigen, which suppresses floral induction antagonizing with florigen, to clarify the role in flowering regulation. From structural and biochemical analysis, anti-florigen was found to form a stable complex with florigen receptor on target promoters and inhibited a formation of florigen-receptor complex. The bindings of florigen and anti-florigen to the receptor were exchangeable, suggesting that the balance of the two proteins is important for determining the timing of floral transition.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] The crystal structure of the plant small GTPase OsRacl reveals its mode of binding to NADPH oxidase.2015

    • Author(s)
      Kosami KI, Ohki I, Nagano M, Furuita F, Sugiki T, Kawano Y, Kawasaki T, Fujiwara T, Nakagawa A, Shimamoto K, Kojima C
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 289 Issue: 41 Pages: 28569-78

    • DOI

      10.1074/jbc.m114.603282

    • Description
      (in press)(印刷中)
    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac12014

    • Author(s)
      Kosami K
    • Journal Title

      Acta Cryst.

      Volume: F70 Issue: 1 Pages: 113-115

    • DOI

      10.1107/s2053230x13033645

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] メチル化リジンの化学シフトと塩橋との相関に関する理論的・実験的研究2014

    • Author(s)
      服部 良一、Jakub Sebera、Vladimir Sychrovsky、古板 恭子、大木  出、池上 貴久、藤原敏道、児嶋 長次郎
    • Journal Title

      日本核磁気共鳴学会機関誌

      Volume: 5 Pages: 56-59

    • Related Report
      2014 Research-status Report
  • [Presentation] 植物のメチル化DNA結合タンパク質の機能構造解析2017

    • Author(s)
      大木出、白川昌宏
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物のメチル化DNA結合タンパク質の構造機能解析2016

    • Author(s)
      尾野有菜、大木出、竹下至、白川昌宏
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市西区)
    • Year and Date
      2016-12-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物のメチル化DNA結合タンパク質の機能構造解析2016

    • Author(s)
      大木出、竹下至、白川昌宏
    • Organizer
      第16回日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市博多区)
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物のメチル化DNA結合タンパク質の機能解析2015

    • Author(s)
      竹下至、大木出、白川昌宏
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市中央区)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report

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Published: 2014-09-09   Modified: 2018-03-22  

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