| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
We constructed the stable bacterial enrichment culture with functions of cellulose degradation and nitrogen fixation from cut grass residues. When a filter paper was added to the culture, paper degradation was observed within 6 days while simultaneously detecting the nitrogen fixation. The extract DNA from the culture was used for the community analysis. The next generation sequencing (NGS) of 16S rRNA genes showed that enrichment culture contained 11 genera as predominant bacterial groups included both nitrogen fixing and cellulose degrading bacteria. Based on the NGS information, we isolated five nitrogen-fixing bacteria, which sequences are corresponded with the major bacterial groups. The isolates were closely affiliated with genera Azoarcus, Pseudomonas (Azomonas), Azospira, Azospirillum and Rhizobium. They are commonly known as endophytic or symbiotic nitrogen-fixing bacteria. Thus, these nitrogen-fixing bacteria suggested to be key member in this bacterial consortia.
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