Elucidation of planteose metabolic pathway in parasitic weeds for their selective control

• Project/Area Number: 26520310
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Food Cycle Research
• Research Institution: Osaka Prefecture University
• Principal Investigator: Okazawa Atsushi 大阪府立大学, 生命環境科学研究科, 准教授 (10294042)
• Project Period (FY): 2014-07-18 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 寄生雑草 / ハマウツボ科 / ストライガ / オロバンキ / プランテオース / ノジリマイシン / 除草剤 / オミクス解析 / 根寄生雑草 / ハマウツボ / ガラクトシダーゼ / インベルターゼ / 選択的除草剤 / ハマウツボ属 / ストライガ属 / 寄生植物 / メタボロミクス

Outline of Final Research Achievements

Parasitic weeds damage agriculture in arid and semi-arid land worldwide and jeopardize global food security. We had conducted metabolome analysis of parasitic weed germination and revealed that planteose metabolic pathway can be promising target for their selective control. In this study, we tried to elucidate enzymes involved in planteose metabolic pathway. Tomato mutants lacking genes involved in planteose biosynthesis were obtained for gene identification. An enzyme catalyzing the first hydrolysis of planteose was successfully elucidated.

Research Products

• [Int'l Joint Research] National Center for Research(Sudan)
• [Int'l Joint Research] ナント大学(フランス)
• [Journal Article] Omics-based exploration for identification of targets for herbicides selective to root parasitic weeds (2017)
  • Author(s): 岡澤 敦司
  • Journal Title: Japanese Journal of Pesticide Science Volume: 42 Issue: 1 Pages: 84-90
  • DOI: 10.1584/jpestics.W17-25
  • NAID: 130005995696
  • ISSN: 2187-0365, 2187-8692
  • Acknowledgement Compliant
• [Journal Article] 根寄生雑草選択的な除草剤の開発に向けて (2016)
  • Author(s): 岡澤 敦司
  • Journal Title: 生産と技術 Volume: 68 Pages: 90-93
  • NAID: 40020824096
  • Open Access / Acknowledgement Compliant
• [Journal Article] 根寄生雑草の発芽におけるプランテオース代謝の役割と防除標的としての可能性 (2015)
  • Author(s): 若林孝俊，岡澤敦司
  • Journal Title: 植物の生長調節 Volume: 50 Pages: 150-155
  • NAID: 110010014909
  • Acknowledgement Compliant
• [Journal Article] Planteose as a storage carbohydrate required for early stage of germination of Orobanche minor and its metabolism as a possible target for selective control (2015)
  • Author(s): Wakabayashi T, Benesh J, Yasumoto S, Akashi T, Aoki T, Harada K, Muranaka S, Bamba T, Fukusaki E, Takeuchi Y, Yoneyama K, Muranaka T, Sugimoto Y and Okazawa A.
  • Journal Title: Journal of Experimental Botany Volume: 印刷中 Issue: 11 Pages: 3085-3097
  • DOI: 10.1093/jxb/erv116
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 代謝解析による寄生雑草防除法の開発 (2014)
  • Author(s): 岡澤敦司，若林孝俊
  • Journal Title: 生物工学会誌 Volume: 92 Pages: 549-552
• [Presentation] 根寄生雑草に特徴的な代謝経路の同定と選択的除草剤への展開 (2017)
  • Author(s): 岡澤 敦司
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2017-03-17
  • Invited
• [Presentation] Metabolism of planteose, a storage carbohydrate in seeds of root parasitic Orobanchaceae (2017)
  • Author(s): Atsushi Okazawa
  • Organizer: 10th International Symposium Exploring the Global Sustainability -Advances in plant biotechnology for agriculture in semi-arid land-
  • Place of Presentation: Osaka University
  • Year and Date: 2017-03-14
  • Int'l Joint Research
• [Presentation] 根寄生雑草ヤセウツボの発芽種子で発現しているαーガラクトシダーゼの機能解析 (2017)
  • Author(s): 馬場敦也，徳永智也，木場康介，小川拓水，杉本幸裕，太田大策，岡澤敦司
  • Organizer: 日本農薬学会第42回大会
  • Place of Presentation: 愛媛大学
  • Year and Date: 2017-03-06
• [Presentation] Selective inhibition of germinaiton of root parasitic weeds by nojirimycin. An inhibitor of planteose metabolism (2017)
  • Author(s): Takatoshi Wakabayashi, Yukihiro Sugimoto, Atsushi Okazawa
  • Organizer: SATREPS 2 Symposium Development of counter measures against Striga to conquer poverty and improve food security in Sudan
  • Place of Presentation: Ministry of Higher Education and Scientific Research Conference Hall, Khartoum, Sudan
  • Int'l Joint Research
• [Presentation] Metabolic study for development of a novel and selective herbicide against root parasitic weeds (2016)
  • Author(s): Atsushi Okazawa
  • Organizer: The 1st China-Japan-Korea Trilateral Joint Symposium on Plant Biotechnolgy
  • Place of Presentation: Wanyilijing Hotel, Guiyang, Guizhou, China
  • Year and Date: 2016-08-03
  • Int'l Joint Research
• [Presentation] 根寄生雑草防除研究へ展開するためのトマト変異体のスクリーニング (2016)
  • Author(s): 岡澤敦司，橋本千里，水川翔太，小川拓水，青木考，太田大策
  • Organizer: 日本農薬学会第 41 回大会
  • Place of Presentation: 島根大学（島根県松江市）
  • Year and Date: 2016-03-17
• [Presentation] オミクス解析による根寄生雑草選択的除草剤の標的探索 (2016)
  • Author(s): 岡澤敦司
  • Organizer: 日本農薬学会第 41 回大会
  • Place of Presentation: 島根大学（島根県松江市）
  • Year and Date: 2016-03-17
  • Invited
• [Presentation] Omics-based exploration for identification of targets for selective control of root parasitic weeds (2016)
  • Author(s): Atsushi Okazawa
  • Organizer: 9th International Symposium Exploring the Global Sustainability -Advances in Plant Biotechnology for Agriculture in Semi-arid Land-
  • Place of Presentation: 大阪大学（大阪府吹田市）
  • Year and Date: 2016-03-07
  • Int'l Joint Research
• [Presentation] 植物代謝研究の応用展開ー根寄生雑草の防除とゲノム編集に関する話題ー (2016)
  • Author(s): 岡澤敦司
  • Organizer: 住友化学株式会社内セミナー
  • Place of Presentation: 住友化学（兵庫県宝塚市）
  • Year and Date: 2016-02-19
  • Invited
• [Presentation] ノーベル平和賞級？猛威をふるう寄生植物による被害の克服を目指して (2016)
  • Author(s): 岡澤敦司
  • Organizer: 智の木協会新年交流会
  • Place of Presentation: 智の木協会（大阪府大阪市）
  • Year and Date: 2016-01-23
  • Invited
• [Presentation] Metabolic analysis toward development of herbicides selective to root parasitic weeds (2016)
  • Author(s): Atsushi Okazawa
  • Organizer: NAIST interdisciplinary workshop Frontiers in Parasitic Plant and Host Interactions
  • Place of Presentation: NAIST Biosicence lecture hall
  • Int'l Joint Research / Invited
• [Presentation] Discovery of novel targets for selective chemical control of root parasitic weeds based on metabolic and transcriptomic analysis of their germination (2015)
  • Author(s): Atsushi Okazawa
  • Organizer: Pacifichem 2015
  • Place of Presentation: Hilton Waikiki Beach, Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 根寄生雑草ヤセウツボの発芽過程における RNA-Seq によるトランスクルプトーム解析 (2015)
  • Author(s): 若林孝俊，村中俊哉，杉本幸裕，岡澤敦司
  • Organizer: 植物化学調節学会第 50 回大会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-10-23
• [Presentation] トマトにおけるプランテオース生合成関連遺伝子の探索 (2015)
  • Author(s): 水川翔太，橋本千里，若林孝俊，小川拓水，青木考，太田大策，岡澤敦司
  • Organizer: 第 32 回日本植物細胞分子生物学会（東京）大会・シンポジウム
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-08-10
• [Presentation] 根寄生雑草ヤセウツボに対する抵抗性因子の探索雨 (2015)
  • Author(s): 橋本千里，小川拓水，青木考，太田大策，岡澤敦司
  • Organizer: 第 32 回日本植物細胞分子生物学会（東京）大会・シンポジウム
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-08-10
• [Presentation] 発芽種子中の代謝経路の理解による根寄生雑草防除への挑戦 (2015)
  • Author(s): 岡澤敦司
  • Organizer: 日本農芸化学会関西支部例会（第 490 回講演会）
  • Place of Presentation: 大阪府立大学（大阪府堺市）
  • Year and Date: 2015-06-04
  • Invited
• [Presentation] 根寄生雑草選択的発芽阻害剤ノジリマイシンがヤセウツボ発芽初期過程の遺伝子発現に及ぼす影響 (2015)
  • Author(s): 若林孝俊，村中俊哉，杉本幸裕，岡澤敦司
  • Organizer: 日本農薬学会第 40 回大会
  • Place of Presentation: 玉川大学（東京都・町田市）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Identification of a novel metabolic target for discovery of selective herbicides for root parasitic weeds (2015)
  • Author(s): Okazawa, A.
  • Organizer: 8th International Symposium Exploring the Global Sustainability
  • Place of Presentation: Osaka University (Suita, Osaka)
  • Year and Date: 2015-03-03 – 2015-03-04
  • Invited
• [Presentation] 食糧の安定供給を目指した根寄生雑草克服技術の開発 (2014)
  • Author(s): 岡澤敦司
  • Organizer: 未来へのバイオ技術勉強会
  • Place of Presentation: 一般財団法人バイオインダストリー協会（東京・中央区八丁堀）
  • Year and Date: 2014-11-13
  • Invited
• [Presentation] Chemical Inhibition of germination based on metabolic knowledge (2014)
  • Author(s): Okazawa, A.
  • Organizer: SATREPS International Symposium on Striga Management
  • Place of Presentation: Khartoum, Sudan
  • Year and Date: 2014-09-15
• [Presentation] 寄生雑草選択的な除草剤の開発に向けた代謝解析 (2014)
  • Author(s): 岡澤敦司
  • Organizer: 第 66 回日本生物工学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2014-09-08 – 2014-09-11
• [Presentation] 放線菌 Streptomyces ficellus による寄生植物発芽種子阻害剤ノジリマイシンの生産 (2014)
  • Author(s): 鈴岡万季，黒野友理香，長澤沙弥，松浦秀幸，岡澤敦司，原田和生，平田收正
  • Organizer: 第 66 回日本生物工学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2014-09-08 – 2014-09-11
• [Presentation] 根寄生雑草発芽阻害剤ノジリマイシンが及ぼすプランテオース代謝経路への影響 (2014)
  • Author(s): 若林孝俊，杉本幸裕，村中俊哉，岡澤敦司
  • Organizer: 第 32 回日本植物細胞分子生物学会（盛岡）大会・シンポジウム
  • Place of Presentation: アイーナいわて県民情報交流センター（岩手県・盛岡市）
  • Year and Date: 2014-08-20 – 2014-08-22
• [Presentation] Study on the effect of nojirimycin on sugar metabolism in germinating seeds for selective control of root parasitic weeds (2014)
  • Author(s): Wakabayashi, T., Yasumoto, S., Akashi, T., Aoki, T., Sugimoto, Y., Ohta, D., Muranak, T., Okazawa, A.
  • Organizer: 13th IUPAC International Congress of Pesticide Chemistry
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2014-08-10 – 2014-08-15
• [Book] Chemical control of root parasitic weeds (Chapter), in "Discovery and Synthesis of Crop Proteiction Products" (2015)
  • Author(s): Okazawa, A., Wakabayashi, T.
  • Total Pages: 467
  • Publisher: American Chemical Society
• [Remarks] 大阪府立大学大学院生命環境科学研究科応用生命科学専攻細胞代謝機能学研究室
  • URL: http://cell-metabolism.sakura.ne.jp
• [Remarks] 大阪府立大学大学院生命環境化学研究科応用生命科学専攻細胞代謝機能学研究室
  • URL: http://cell-metabolism.sakura.ne.jp