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Construction of DNA computer using artificial cell circuit

Research Project

Project/Area Number 26540160
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Life / Health / Medical informatics
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Kawano Ryuji  東京農工大学, 工学(系)研究科(研究院), 准教授 (90401702)

Co-Investigator(Kenkyū-buntansha) TAKINOUE Masahiro  東京工業大学, 大学院情報理工学院, 准教授 (20511249)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsナノポア / DNA演算 / マイクロデバイス / DNAコンピューティング / 脂質二分子膜 / ドロップレット / マイクロ流体
Outline of Final Research Achievements

The goal of this project is the construction of DNA computing using artificial cell membrane with nanopore. As the result of two years struggling, we have integrated DNA computing system into the droplet networks in the microdevices. These results have been gave publicity to several conferences and academic journals.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (15 results)

All 2016 2015 2014 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 5 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Remarks (1 results)

  • [Journal Article] Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array2016

    • Author(s)
      M. Ohara, Y. Sekiya, R. Kawano
    • Journal Title

      Electrochemistry

      Volume: accepted

    • NAID

      130005150414

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Logic Gate Operation by DNA Translocation through Biological Nanopores2016

    • Author(s)
      †H. Yasuga,† R. Kawano, M. Takinoue, T. Tsuji, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi (†equal contribution)
    • Journal Title

      PLoS One

      Volume: 11 Issue: 2 Pages: e0149667-e0149667

    • DOI

      10.1371/journal.pone.0149667

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Logic Gate Operation using Three-way Junction DNA and Biological Nanopore2015

    • Author(s)
      M. Ohara and R. Kawano
    • Journal Title

      Proceedings of MicroTAS

      Volume: 1 Pages: 1493-1495

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] ドロップレット型人工細胞膜によるチャネル膜タンパク質計測2015

    • Author(s)
      川野竜司
    • Journal Title

      生物物理

      Volume: 通巻318号 Pages: 077-080

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Planar Lipid Bilayer Formation Using Droplet Contact Method and Its Applications2015

    • Author(s)
      川野竜司
    • Journal Title

      Seibutsu Butsuri

      Volume: 55 Issue: 2 Pages: 077-080

    • DOI

      10.2142/biophys.55.077

    • NAID

      130005061221

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] DNA/RNA Computing with Biological Nanopore in Droplets System: AND Operation Using RNA Polymerization2014

    • Author(s)
      M. Ohara, M. Takinoue and R. Kawano
    • Journal Title

      Proceedings of MicroTAS

      Volume: 1 Pages: 1790-1792

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Parallel Lipid Bilayer Formation by Microfabrication and Its Applications2016

    • Author(s)
      R. Kawano
    • Organizer
      PITTCON 2016
    • Place of Presentation
      Atlanta, US
    • Year and Date
      2016-03-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fusion between Microfabrication and Biomaterials: Application for Artificial Cell Membranes and Biomimetic Microfluidics2015

    • Author(s)
      R. Kawano
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii, US
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Logic Gate Operation using Three-way Junction DNA and Biological Nanopore2015

    • Author(s)
      M. Ohara and R. Kawano
    • Organizer
      MicroTAS2015
    • Place of Presentation
      Gyeongju, Korea
    • Year and Date
      2015-10-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 液滴界面に形成した脂質二分子膜によるチャネル膜タンパク質の一分子計測2015

    • Author(s)
      川野竜司
    • Organizer
      第64回高分子討論会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] マイクロ加工・流体技術を用いた人工細胞膜システムの開発2015

    • Author(s)
      川野竜司
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール(徳島市)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Construction of DNA/RNA logic gates with biological nanopores2014

    • Author(s)
      R. Kawano
    • Organizer
      細胞を作る会7.0
    • Place of Presentation
      東京大学
    • Year and Date
      2014-11-13 – 2014-11-14
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] DNA/RNA Computing with Biological Nanopore in Droplets System: AND Operation Using RNA Polymerization2014

    • Author(s)
      M. Ohara, M. Takinoue and R. Kawano
    • Organizer
      The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Texas, US
    • Year and Date
      2014-10-26 – 2014-10-30
    • Related Report
      2014 Research-status Report
  • [Presentation] α-hemolysin及びT7 RNA polymeraseを用いたDNA/RNAロジックゲートの実現2014

    • Author(s)
      大原正行、瀧ノ上正浩、川野竜司
    • Organizer
      第52回日本生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Remarks] 川野研究室

    • URL

      http://web.tuat.ac.jp/~rjkawano/index.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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