| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
For better understanding of the dynamics of organic matter in the ocean interior, particulate organic matter (POM) in oceanic surface water is a key material as a starting material in food chain and biological carbon pump. In our previous studies we reported that most of POM existed as glycopeptide. The details of molecular-level structures including sugar chains are unknown. In this study, we applied various chemical fractionation and the electrophoretic separation for sugar chains to the POM samples and tried to clarify chemical characteristics of the glycopeptides. Some band and background were detected on electrophoretogram. The bands were sugar chains with unmodified reduced terminus and consistent with 3-13 glucose polymer. The background sugar chains had broad degree of polymerization. These visible sugar chains accounted for only 1.6% of sugar of POM. It was suggested that most of sugars in POM were large polymers which could not migrate in the gel for the electrophoresis.
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